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山东大学学报(医学版) ›› 2014, Vol. 52 ›› Issue (2): 48-51.doi: 10.6040/j.issn.1671-7554.0.2013.260

• 基础医学 • 上一篇    下一篇

孤独症大鼠前额叶皮质神经元凋亡相关蛋白的表达

文敏1,赵晶2,鲍星星3,邓成敏4,童雪涛3   

  1. 1.贵阳医学院人体解剖学教研室, 贵阳 550004; 2.贵阳市第二人民医院神经内科, 贵阳 550004;
    3.贵阳医学院附属医院儿科, 贵阳 550004; 4.贵阳医学院生物技术教研室, 贵阳 550004
  • 收稿日期:2013-04-15 出版日期:2014-02-10 发布日期:2014-02-10
  • 通讯作者: 童雪涛, E-mail:1115393686 @qq.com
  • 基金资助:

    贵州市科技计划项目[筑科合同(2011103)18号];贵州省科技攻关计划课题[黔科合SY(2010)3082号]

Expression of the apoptosis-related protein in prefrontal cortex in a rat model of autism

WEN Min1, ZHAO Jing2, BAO Xing-xing3, DENG Cheng-min4, TONG Xue-tao3   

  1. 1. Department of Anatomy, Guiyang Medical University, Guiyang 550004, China;
    2. Department of Neurology, the Second People′s Hospital, Guiyang 550004, China;
    3. Department of Pediatrics, Affiliated Hospital of Guiyang Medical University, Guiyang 550004, China;
    4. Department of Biological Technology, Guiyang Medical University, Guiyang 550004, China
  • Received:2013-04-15 Online:2014-02-10 Published:2014-02-10

摘要:

目的  观察孤独症大鼠前额叶皮质神经元凋亡相关蛋白Caspase-3和Bcl-2表达的变化,探讨细胞凋亡在孤独症发病中的作用。方法   健康繁殖期Wistar雌鼠20只,体质量250~260g,随机选取10只在E12.5腹腔注射丙戊酸钠(VPA),其子代为孤独症模型组;其余10只在E12.5腹腔注射生理盐水,其子代为对照组。通过比较负向性实验、自梳理实验验证模型是否成功;Western blotting方法   对比对照组与孤独症模型组大鼠P42前额叶皮质凋亡相关蛋白Caspase-3和Bcl-2表达的变化。结果   成功建立孤独症动物模型。与对照组比较,孤独症模型组大鼠旋转调头时间显著延长(P<0.05),理毛时间显著增加(P<0.05);P42前额叶皮质凋亡相关蛋白Caspase-3表达显著降低(P <0.05),Bcl-2表达显著升高(P<0.05)。结论    孤独症大鼠P42前额叶皮质凋亡受到抑制,提示调节凋亡可能是一个潜在的孤独症治疗策略。

关键词: 凋亡, 孤独症, Caspase-3, Bcl-2

Abstract:

Objective   To investigate the change of apoptosis-related protein Caspase-3 and Bcl-2 and effect of apoptosis in the rat model of autism. Methods  Ten female Wistar rats (250-260 g) out of 20 were selected randomly to receive a single intraperitoneal injection of sodium valproate (VPA) at the pregnancy day of 12.5, whose offspring were defined as the autism model group. The rest 10 female Wistar rats received the intraperitoneal injection of saline, whose offspring were defined as the control group. To confirm whether the animal model was successfully established, the negative geotaxis test and self-grooming test were compared between the autism model group and control group. Western blotting was used to detect the expressions of Caspase-3 and Bcl-2 in prefrontal cortex in the two groups. Results  The animal model of autism was successfully established. Compared with the control group, the negative geotaxis time and the cumulative self-grooming time in the autism model group significantly increased (P<0.05); the expression of Caspase-3 decreased while Bcl-2 in prefrontal cortex incresed(all P<0.05) in the autism model group. Conclusion  The apoptosis is inhibited in prefrontal cortex of autism, which suggests that the modulation of apoptosis might be a potential therapeutic strategy for autism.

Key words: Caspase-3, Autism, Bcl-2, Apoptosis

中图分类号: 

  • R729
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