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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (9): 11-14.doi: 10.6040/j.issn.1671-7554.0.2014.097

• 基础医学 • 上一篇    下一篇

大麻素受体1与FAK在血吸虫肝纤维化小鼠肝组织中的表达

欧阳兵1, 彭忠田2, 王培3   

  1. 1. 南昌市第九医院感染一科, 江西 南昌 330002;
    2. 南华大学附属第一医院感染科, 湖南 衡阳 421001;
    3. 怀化市第一人民医院感染科, 湖南 怀化 418000
  • 收稿日期:2014-02-26 修回日期:2014-09-04 出版日期:2014-09-10 发布日期:2014-09-10

Expression and significance of cannabinoid receptor 1 and FAK in hepatic tissues of schistosomal hepatic fibrosis mice

OUYANG Bing1, PENG Zhongtian2, WANG Pei3   

  1. 1. Department of Infectious Diseases, Ninth Hospital of Nanchang, Nanchang 330002, Jiangxi, China;
    2. Department of Infectious Diseases, First Affiliated Hospital of University of South China, Hengyang 421001, Hu'nan, China;
    3. Department of Infectious Diseases, First People's Hospital of Huaihua City, Huaihua 418000, Hu'nan, China
  • Received:2014-02-26 Revised:2014-09-04 Online:2014-09-10 Published:2014-09-10
  • Contact: 彭忠田。E-mail:hncspzt@yahoo.com.cn E-mail:hncspzt@yahoo.com.cn

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摘要： 目的 探讨大麻素受体1（CB1）与黏着斑激酶（FAK）在血吸虫肝纤维化小鼠肝组织中的表达。方法 将32只昆明小鼠分为正常组（n=10）和模型组（n=22），两组动物均普通喂养，8周后处死取肝组织。模型组小鼠采用腹壁贴附法建立血吸虫肝纤维化小鼠模型。根据纤维化程度将模型组分为Ⅰ级肝纤维化组（n=4）、Ⅱ级肝纤维化组（n=8）和Ⅲ级肝纤维化组（n=10）。HE染色观察病理变化，Masson染色观察肝纤维化程度，免疫组织化学法检测不同纤维化组CB1的表达，RT-PCR法检测各组CB1 mRNA及FAK mRNA的表达。结果 模型组可见明显虫卵结节及纤维化改变；不同纤维化组均可见CB1、CB1 mRNA及FAK mRNA表达，且随着肝脏纤维化程度的增加，CB1、CB1 mRNA及FAK mRNA的表达增加（P<0.05）。结论 CB1和FAK参与了血吸虫肝纤维化的发生与发展。

关键词: 大麻素受体1, 肝纤维化, 小鼠, 黏着斑激酶

Abstract: Objective To investigate the expression and significance of cannabinoid receptor 1 (CB1) and focal adhesion kinase (FAK) in hepatic tissues of schistosomal hepatic fibrosis mice. Methods A total of 32 Kunming mice were randomly divided into two groups: normal control group (n=10) and model group (n=22). The schistosome-induced liver fibrosis models were established by attaching cercaria to the skin on the ventral side of the mice and allowing infection to occur via direct penetration. All mice were raised normally, and at week 8 all mice were sacrificed to gain hepatic tissue samples. Mice in the model group were divided into 3 subgroups according to the severity of hepatic fibrosis. The pathological changes were determined by HE staining, the degrees of fibrosis were examined by Masson staining, the expression of CB1 was detected by immunohistochemical method, and the expressions of FAK mRNA and CB1 mRNA were tested by reverse transcription polymerase chain reaction (RT-PCR). Results In the model group, significant schistosome egg granuloma and hepatic fibrosis were observed, and CB1, CB1 mRNA and FAK mRNA were detected in all subgroups. Besides, with the aggravation of fibrosis, the expressions of CB1, CB1 mRNA and FAK mRNA increased. Conclusion CB1 and FAK play an important role in the genesis and progression of schistosomal hepatic fibrosis.

Key words: Focal adhesion kinase, Schistosomal hepatic fibrosis, Cannabinoid receptor-1, Mice

中图分类号: 

  • R381
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