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Role of rapamycin on cultured human Tenon's capsule fibroblasts

ZHAO Jie, CAI Ke-li   

  1. Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2006-07-20 Revised:1900-01-01 Online:2007-06-24 Published:2007-06-24
  • Contact: CAI Ke-li

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Abstract: Objective: To explore the effect of rapamycin on the proliferation and differentiation of human Tenon′s capsule fibroblasts in cell cycles. Methods: The cultured fibroblasts were treated in DMEM medium containing various concentrations of rapamycin for 24 hours. The growth of fibroblasts was measured by a methylthiazlyl tetrazolium (MTT) assay. The flow cytometry method was used to evaluate the cell cycles. Results: Proliferation of fibroblasts were suppressed by rapamycin from a dose of 10?ng/ml to 160ng/ml(P＜0.05)． The cell growth inhibition rate treated with rapamycin concentrations of 10ng/ml, 20ng/ml, 40ng/ml, 80ng/ml, and 160ng/ml were 9.32%, 22.55%, 41.42%, 42.25%, and 43.57% respectively. The cell population was significantly increased at the G0/Gl phase from 41.76% to 44.19% and decreased at the S phase from 45.42% to 40.18% after the rapamycin treatment． Conclusion: Rapamycin is a potential inhibitor that blocks the proliferation of human Tenon′s capsule fibroblasts at the Gl restriction point．

Key words: Rapamycin, Tenon′s capsule, Fibroblasts, Glaucoma

CLC Number: 

  • R775
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