JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (12): 58-61.doi: 10.6040/j.issn.1671-7554.0.2016.464

Previous Articles     Next Articles

Role of vascular endothelial cell activation in the pathogenesis of post infectious irritable bowel syndrome

LI Qian1, DAI Taoli1, HAN Wei1, GUO Chenghao2   

  1. 1. Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Pathology, Medical School of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-04-25 Online:2016-12-10 Published:2016-12-10

PDF (PC)

120

Abstract: Objective To investigate the morphological changes of vascular endothelial cells as well as changes in the level of plasma endothelin(ET)and nitric oxide(NO)in patients with post infectious irritable bowel syndrome(PI-IBS), in order to clarify the possible role of vascular endothelial cell activation in the pathogenesis of PI-IBS. Methods The mucosa biopsy samples and plasma were obtained from 28 PI-IBS patients and 12 healthy controls during the same period. The morphological changes of vascular endothelial cells were observed with light microscope. The plasma ET and NO levels in both groups were measured with radioimmunoassay and nitrate reductase method. Results There were more significant morphological changes in vascular endothelial cells in the PI-IBS patients than in the controls. The plasma ET and NO levels of the PI-IBS patients were(55.49±13.49)ng/L and(75.90±16.52)μmol/L, respectively, which were significantly higher than those of the controls［(43.40±13.69)ng/L and(62.48±15.63)μmol/L, both P<0.05］. Conclusion The vascular endothelial cell activation may be closely related to the pathogenesis of PI-IBS.

Key words: Vascular endothelial cell activation, Endothelin, Nitric oxide, Post infectious irritable bowel syndrome, Morphology

CLC Number: 

  • R574
[1] Serghini M, Karoui S, Boubaker J, et al. Post-infectious irritable bowel syndrome[J]. Tunis Med, 2012, 90(3): 205-213.
[2] Spiller RC, Jenkins D, Thornley JP, et al. Increased rectal mucosal enteroendocrine cells, T lymphocytes, and increased gut permeability following acute Campylobacter enteritis and in post-dysenteric irritable bowel syndrome[J]. Gut, 2000, 47(6): 804-811.
[3] Sundin J, Rangel I, Kumawat AK, et al. Aberrant mucosal lymphocyte number and subsets in the colon of post-infectious IBS patients[J]. Scand J Gastroenterol, 2014, 49(9): 1068-1075.
[4] Cotton JA, Beatty JK, Buret AG. Host parasite interactions and pathophysiology in Giardia infections[J]. Int J Parasitol, 2011, 41(9): 925-933.
[5] Martínez C, González-Castro A, Vicario M, et al. Cellular and molecular basis of intestinal barrier dysfunction in the irritable bowel syndrome[J]. Gut Liver, 2012, 6(3): 305-315.
[6] Zhou Q, Zhang B, Verne GN. Intestinal membrane permeability and hypersensitivity in the irritable bowel syndrome[J]. Pain, 2009, 146(1-2): 41-46.
[7] Kwok W, Clemens MG. Rho-kinase activation contributes to Lps-induced impairment of endothelial nitric oxide synthase activation by endothelin-1 in cultured hepatic sinusoidal endothelial cells[J]. Shock, 2014, 42(6): 554-561.
[8] Kim YJ, Koo TY, Yang WS, et al. Activation of spleen tyrosine kinase is required for TNF-α-induced endothelin-1 upregulation in human aortic endothelial cells[J]. FEBS Lett, 2012, 586(6): 818-826.
[9] Zhang J, Defelice AF, Hanig JP, et al. Biomarkers of endothelial cell activation serve as potential surrogate markers for drug-induced vascular injury[J]. Toxicol Pathol, 2010, 38(6): 856-871.
[10] Dai C, Guandalini S, Zhao DH, et al. Antinociceptive effect of VSL#3 on visceral hypersensitivity in a rat model of irritable bowel syndrome: a possible action through nitric oxide pathway and enhance barrier function[J]. Mol Cell Biochem, 2012, 362(1-2): 43-53.
[11] Rapoport RM. Acute nitric oxide synthase inhibition and endothelin-1-dependent arterial pressure elevation[J]. Front Pharmacol, 2014, 5: 57. doi: 10.3389/fphar.2014.00057.
[12] Saini V, Bhatnagar MK, Bhattacharjee J. Association of endothelial dysfunction with endothelin, nitric oxide and eNOS Glu298Asp gene polymorphism in coronary artery disease[J]. Dis Markers, 2011, 31(4): 215-222.
[13] 陈忠龙, 吴小南. 肠黏膜屏障与胃肠疾病[J]. 海峡预防医学杂志, 2007, 13(2): 31-33.
[14] Dunlop SP, Hebden J, Campbell E, et al. Abnormal intestinal permeability in subgroups of diarrhea-predominant irritable bowel syndromes[J]. Am J Gastroenterol,2006, 101(6): 1288-1294.
[15] Vivinus-Nébot M, Dainese R, Anty R, et al. Combination of allergic factors can worsen diarrheic irritable bowel syndrome: role of barrier defects and mast cells[J]. Am J Gastroenterol 2012, 107(1): 75-81.
[16] Wang H, Gong J, Wang W, et al. Are there any different effects of Bifidobacterium, Lactobacillus and Streptococcus on intestinal sensation, barrier function and intestinal immunity in PI-IBS mouse model?[J]. PLoS One, 2014, 9(3): e90153. doi: 10.1371/journal.pone.0090153.
[17] Halliez MC, Motta JP, Feener TD, et al. Giardia duodenalis induces paracellular bacterial translocation and causes postinfectious visceral hypersensitivity[J]. Am J Physiol Gastrointest Liver Physiol, 2016, 310(8): G574-585.
[1] LIU Lin, LIU Chunhong, WANG Dexiang, WU Jinxiang, ZHAO Jiping, LIU Tian, ZHANG Yuanyuan, WANG Junfei, LIU Yahui, CAO Liuzhao, DONG Liang. Estimation on small airway function in asthmatic patients with fractional exhaled nitric oxide and impulse oscillometry [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 78-83.
[2] LI Xin, WANG Gongming, WANG Hong, WANG Yan, ZHANG Ligong, ZHANG Le, LIU Bei, ZHANG Mengyuan. Inducible nitric oxide synthase contributes to the delayed cardioprotection of ischemic postconditioning in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(2): 16-20.
[3] PAN Yanyan, SUN Yongchao, ZHAO Cuifen, KONG Qingyu. Clinical efficacy of bosentan in the treatment of babies with congenital heart disease complicated with pulmonary hypertension [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(2): 53-56.
[4] LIU Huimin, LIU Deng, LI Xiaoyu, ZOU Shufeng, JIANG Limin, LI Yuhuan. Effect of Lobelia alkaloids on endothelin-1 signal pathway in a rat model of pulmonary arterial hypertension [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 1-4.
[5] LIU Binyu, LIU Binyan, XING Yanxia, HE Yinfei, XU Xin. Experimental study of platelet-rich plasma on the vascularization in skull defects [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 27-33.
[6] Gleniece Irving, WANG Shuo, WANG Hui, GUO Ying, JIANG Lulu, ZHAO Xiulan, XIE Keqin. Effects of carbon disulfide on the learning ability of rats and its underlying mechanisms [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(10): 82-86.
[7] FU Qingyuan, DAI Guiqiang, ZHANG Song, AN Hongchun, LIANG Shimin. Effect of panax quinquefolium saponin from stems and leaves on endothelin-1, vascular endothelial growth factor and infarct area in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 30-33.
[8] SHAO Haigang, WANG Xuan, WANG Qing. Anatomy of the root canal system of mandibular first premolar in population of Shandong Province [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 85-89.
[9] CHENG Longfeng1, PAN Yan1, LIU Shangming2, WANG Liyan2, ZHANG Xiumei3, XU Hongyan4, LI Hongyu5. Inducible nitric oxide synthase expression and protection of hydroxysafflor yellow A in the prefrontal cortex neurons of lymphostatic encephalopathy rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(6): 7-11.
[10] MICHAEL M. P. NG'WANAMASELE, HAN Yanzhao, ZHANG Fenghe, TONG Dongdong, LI Liang, CHENG Huijuan, WANG Chunling, LIU Dongxu. Three dimensional registration method to evaluate the upper airway morphologic changes after mandibular setback surgery [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(10): 61-65,71.
[11] LIU Jing-yuan, ZHANG Xiao, AN Jie. Influence of different chemical irrigants on the micropush-out bond strength of an adhesive system used to cement glass fiber posts to root dentin [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(4): 30-33.
[12] DU Lin-na1， GUO Jing2. Comparison of responders and nonresponders to oral appliance for
the treatment of obstructive sleep apnea syndrome [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(8): 120-123.
[13] SUN Yong-bo, TAN Tue-hou. Animal experiment study in Wister rat′s ischemic myocardia preconditioning with NO relativity [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 42-.
[14] XU Nuo, JIANG Junmei， FENG Shanshan, WANG Guanhua, MENG Mei, YIN Xiaoyan. Effect of endogenous NO on expression of HDAC4 in a  hepatocellular carcinoma model of nude mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(8): 48-51.
[15] ZHOU Dong-zhi, LIU Wen, LI Yue-jun, XIA Xin-xin, WANG Yu, LIU Yong-hui. Therapeutic effect and anti-free radical injury of  emodin on acute radiation enteritis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 76-79.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd