JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (2): 6-11.doi: 10.6040/j.issn.1671-7554.0.2014.658

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Protective effect and possible mechanism of losartan on endotoxin-induced acute lung injury in rats

LIU Leilei, JU Yunfei, XU Wenfei, JU Yuanrong   

  1. Department of Surgical Intensive Care Unit, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250012, Shandong, China
  • Received:2014-09-28 Online:2015-02-10 Published:2015-02-10

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Abstract: Objective To investigate the protective effect and possible mechanism of losartan, an angiotensin Ⅱ (AngⅡ) receptor antagonist, on endotoxin-induced acute lung injury (ALI) in rats. Methods Fifty male Wistar rats were randomly divided into four groups: control group, lipopolysaccharide (LPS) group, LPS+losartan group, and LPS+losartan+A779 group. ALI models of rats were established by intravenous injection of LPS under anesthesia state then intervented with losartan or A779, which is an antagonist of angiotensin 1-7[Ang (1-7)]. The former three groups were observed at 1, 4 and 6 h and the fourth group was observed at 4 h, with 5 rats at each time point. Proteins derived from bronchial alveolar lavage fluid (BALF) and serum were measured by BCA method. The pathological changes in the middle of the right lung tissue were observed with hematoxylin-eosin (HE) staining and the pathological scores were obtained. The contents of AngII and Ang (1-7) in serum were assessed by ELISA. Results The characteristiclly pathological changes in rats could be observed under optical microscope. Compared with control group, the contents of serum AngII at 1 h and 4 h in LPS group increased (P<0.01); the content of serum Ang (1-7) at 1 h in LPS group decresed, butat 4 h increased (all P<0.01). Compared with LPS group, the lung injury alleviated in LPS+losartan group and the pathological score decreased (P<0.01). Conclusion Losartan has a protective effect, which depends largely on regulating the level of Ang (1-7), on endotoxin-induced ALI in rats.

Key words: Angiotensin II receptor antagonist, losartan, Acute lung injury/Acute respiratory distress syndrome, Angiotensin 1-7

CLC Number: 

  • R563
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