JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (7): 32-36.doi: 10.6040/j.issn.1671-7554.0.2013.702

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Killing effect of apoptotic-tumor-cell-loaded dendritic cells on laryngeal carcinoma cells

ZHANG Min1, WANG Xuefeng2   

  1. 1. Liaoning Medical University, Jinzhou 121001, Liaoning, China;
    2. Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning, China
  • Received:2013-11-23 Revised:2014-06-10 Online:2014-07-10 Published:2014-07-10

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Abstract: Objective To explore the killing effect of apoptotic-tumor-cell-loaded dendritic cells (DCs) on the laryngeal carcinoma cells. Methods DCs from peripheral blood monocytes were induced by recombinant human interleukin-4 (rhIL-4), recombinant human granulocyte/macrophage colony-stimulating factor (rhGM-CSF) and tumor necrosis factor (TNF-α) in vitro, then were divided into the apoptotic laryngeal carcinoma cells group, freeze-thaw cells group and untreated cells group 6 days later. After continuous culture of DCs for 4h, the expressions of the surface markers of DCs were detected by flow cytometry, the level of Interferon-γ (IFN-γ) was measured by ELISA, and the proliferation ability and killing effect of cytotoxic T lymphocyte (CTL) stimulated by DCs were evaluated by MTT. Results Compared with other groups, apoptotic laryngeal carcinoma cells group could enhance the expression of the surface markers CD83, CD86, HLA-DR and IFN-γ(P<0.05), and the proliferation ability and killing effect of CTL increased (P<0.05). Conclusion Apoptotic carcinoma cells can promote the differentiation and maturation of DCs, and induce effective and specific immune response against laryngeal carcinoma.

Key words: Dendritic cell, Laryngeal carcinoma cell, Cisplatin, Apoptotic carcinoma cells

CLC Number: 

  • R730.51
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