Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (9): 140-147.doi: 10.6040/j.issn.1671-7554.0.2021.1064

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Identification of proteins in hypopharyngeal squamous cell carcinoma by mass spectrometry and screening of prognostic target molecules

CHEN Lixiao, YING Xinjiang, CHEN Xinwei, WANG Fei, SUN Zhenfeng, DONG Pin   

  1. Department of Otorhinolaryngology Head and Neck Surgery, Shanghai General Hospital, Shanghai 200080, China
  • Published:2021-10-15

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Abstract: Objective To screen specific target molecules related to the prognosis of advanced hypopharyngeal squamous cell carcinoma using non-data dependent acquisition(DIA)proteomics technology. Methods A total of 5 pairs of advanced hypopharyngeal squamous cell carcinoma and adjacent tissues diagnosed in Shanghai General Hospital during Jan. 2016 and Sep. 2018 were selected. The protein tissues were lysed and ground. Protein electrophoresis, tissue enzymatic hydrolysis, peptide labeling, graded labeling, and high-performance liquid chromatography were performed. Mass spectrometry was performed with Q-Exactive mass spectrometer. Quantitative analysis and bioinformatics analysis were conducted to screen prognosis protein molecules. Quantitative analysis and bioinformatics analysis were used to screen prognosis related protein molecules. Results A proteomics database was established, including 7,395 proteins, 63,190 peptide segments, and 79 differentially expressed proteins(36 up-regulated and 43 down-regulated). After GO and KEGG enrichment, PPI protein network interaction and prognosis analysis, the key protein MPRS5 was selected as the specific target molecule for the prognosis of advanced hypopharyngeal squamous cell carcinoma. Conclusion MRPS5 may become a prognostic target molecule for hypopharyngeal squamous cell carcinoma.

Key words: Hypopharyngeal squamous cell carcinoma, Proteomics, Non-data dependent acquisition, Prognostic factors

CLC Number: 

  • R739.63
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