Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (11): 34-41.doi: 10.6040/j.issn.1671-7554.0.2019.1048

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Clinical application of the fully automatic chemiluminescence to detect interferon-γ test in diagnosis of tuberculosis

LI Xiaofei, LIU Hongwei, ZI Min, LV Songqin, LUO Feng, QIU Wan, HUANG Shan   

  1. Department of Laboratory Medicine, Kunming Third Peoples Hospital, Kunming 650041, Yunnan, China
  • Published:2022-09-27

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Abstract: Objective To explore the clinical application value of the fully automatic chemiluminescence to detect interferon-γ test in diagnosis of tuberculosis. Methods To compare the diagnostic performance of the fully automatic chemiluminescence to detect interferon-γ test and T-cell spot of Tuberculosis(T-SPOT.TB)test in diagnosis of tuberculosis.The tuberculosis group(n>150), non-tuberculosis group easily confused with tuberculosis group(n>100), and healthy controls(n>150)were enrolled in the prospective and synchronous blind study. The diagnostic values of the two tests were compared with receiver operator characteristic(ROC)curve, and consistency of the results was evaluated with Kappa test. Results A total of 400 samples were collected, and 364 were valid, including 141 in tuberculosis group, 43 in non-tuberculosis group, and 180 healthy controls. The area under ROC curve(AUC)of the fully automatic chemiluminescence to detect interferon-γ test was 0.897, AUC of T-SPOT.TB test ［early secreted antigenic target-6(ESAT-6)hole and culture filtrate protein-10(CFP-10)hole］ was 0.884 and 0.879, respectively, and Kappa value was about 0.75. Conclusion The results of the fully automatic chemiluminescence to detect interferon-γ test and T-SPOT.TB test are consistent in diagnosis of tuberculosis. As the fully automatic chemiluminescence to detect interferon-γ test is automatically operated, it will have better clinical application value.

Key words: Chemiluminescence immunoassay, Fully Automatic, Tuberculosis, Interferon-gamma release assay, Enzyme-linked immunospot assay

CLC Number: 

  • R378.91
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