JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (7): 38-42.doi: 10.6040/j.issn.1671-7554.0.2016.856

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Preliminary study of tissue distribution and safety of HSV-2 DNA vaccine in mice

JIAO Fengping1, ZHANG Haitao2, ZHAO Yinghui1, TIAN Zhaoju1, YANG Shulin1   

  1. 1. Department of Clinical Microbiology, School of Public Health, Taishan Medical University, Taian 271016, Shangdong, China;
    2. Department of Neonatology, Maternal and Child Care Service Centre, Taian 271016, Shangdong, China
  • Received:2016-07-15 Online:2017-07-10 Published:2017-07-10

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Abstract: Objective To investigate preliminarily the tissue distribution and safety of HSV-2 DNA vaccine pc-P6-gBCTL-TBK-1 in mice. Methods The recombinant plasmid pc-P6-gBCTL-TBK-1 was injected into BALB/c mice with 100 μg per mouse for three times at every other week. Heart, liver, spleen, kidney, lung, brain, blood and muscle tissues of immune mice were collected at 24 h, 7 d, 21 d, 35 d and 49 d after inoculation, then total DNAs of tissues were extracted, and the tissue distribution of plasmid DNA and the possibility of integration with host genome were detected by PCR assay. Results At 24 h, 7 d, 21 d and 35 d after the last immunization, the plasmid DNA was detected in heart, liver, spleen, kidney, lung, brain and muscle tissues. At 49 d after the last immunization, the plasmid DNA was detected only in muscle tissue. The gel-purified genomic DNA was detected negative by PCR assay. Conclusion The plasmid pc-P6-gBCTL-TBK-1 can spread quickly to the heart, liver, spleen, kidney, lung, brain, blood and muscle tissues of the mice. With the extention of time, the plasmid DNA only distributes in muscle tissue of vaccination, and the DNA does not integrate with the host genome, which suggests that the plasmid is safe.

Key words: Herpes simplex virus type 2, Tissue distribution, Safety, DNA vaccine

CLC Number: 

  • R373.11
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