JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (7): 31-37.doi: 10.6040/j.issn.1671-7554.0.2017.198

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Effects of carbon monoxide releasing molecule-3 on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells

LI Jingyuan1, SONG Ling2, LIN Song3, SONG Hui4   

  1. 1. Department of Oral Mucosal Diseases, Stomatological Hospital of Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, Shandong, China;
    2. Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266011, Shandong, China;
    3. Department of Pediatric Dentistry, Jinan Stomatological Hospital, Jinan 250001, Shandong, China;
    4. Department of VIP Clinic, Stomatological Hospital of Shandong University, Shandong Provincial Key Laboratory ofOral Tissue Regeneration, Jinan 250012, Shandong, China
  • Received:2017-03-07 Online:2017-07-10 Published:2017-07-10

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Abstract: Objective To investigate the effects of carbon monoxide releasing molecule-3(CORM-3)on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells(BMSCs). Methods The BMSCs were divided into 2 groups, group A and group B. Group A was subdivided into osteogenic group, 100 μmol/L CORM-3 group, 200 μmol/L CORM-3 group, 400 μmol/L CORM-3 group and control group. Group B was subdivided into osteogenic 山 东 大 学 学 报 (医 学 版)55卷7期 -李景媛,等.不同浓度一氧化碳释放分子-3对大鼠骨髓间充质干细胞成骨分化的影响 \=-group, 100 μmol/L CORM-3-osteogenic group, 200 μmol/L CORM-3-osteogenic group, 400 μmol/L CORM-3-osteogenic group and control group. The mRNA and protein expressions of Runx2 and OPN were detected with Real-time PCR and Western blotting. The cell mineralization was determined with alizarin red staining. Data obtained were analyzed with SPSS 19.0 software. Results CORM-3 at concentrations of 100 μmol/L and 200 μmol/L could significantly promote the proliferation of BMSCs(P=0.034, P<0.001). The mRNA expressions of Runx2 and OPN in 200 μmol/L and 400 μmol/L CORM-3-osteogenic groups were significantly higher than those in the osteogenic group on day 7(Runx2: P=0.005, P=0.006; OPN: P=0.010, P=0.028). Meanwhile, the protein expressions of Runx2 and OPN on day 7 and cell mineralization on day 21 in 200 μmol/L and 400 μmol/L CORM-3-osteogenic groups were enhanced compared with the osteogenic group, and the 200 μmol/L group had better results than the 400 μmol/L group. Conclusion CORM-3 may promote osteogenic differentiation of BMSCs and 200 μmol/L is the suitable concentration.

Key words: Carbon monoxide releasing molecules-3, Osteogenic differentiation, Bone marrow-derived mesenchymal stem cells, Runt-related transcription factor 2, Osteopontin

CLC Number: 

  • R781.4
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