JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (3): 14-18.doi: 10.6040/j.issn.1671-7554.0.2015.833

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Effect of tumor associated macrophages on the expression of c-Met in hepatocellular carcinoma cells

ZONG Zhaoyun1, LI Xia2, HAN Zhenlong3, WANG Xianteng1, GUO Chun1, ZHANG Lining1, SHI Yongyu1   

  1. 1. Department of Immunology, School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Oncology, Jinan First Peoples Hospital, Jinan 250011, Shandong, China;
    3. Department of Clinical Laboratory, General Hospital of Police Officer, Jinan 250002, Shandong, China
  • Received:2015-09-01 Online:2016-03-10 Published:2016-03-10

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Abstract: Objective To explore the molecular mechanism of c-Met expression in hepatocellular carcinoma cells(HCC)influenced by tumor associated macrophages. Methods HCC cells were cocultured with macrophages derived from THP-1 cells. The expression of c-Met in HCC cells was detected at mRNA level by RT-PCR and protein level by flow cytometry. The migration of HCC cells was detected by Transwell cell migration test. Results The expression of c-Met in HCC cells was upregulated by the macrophages at both mRNA and protein levels. After HCC cells were cocultured with macrophages, the migration was enhanced in the presence of human recombinant protein hepatocyte growth factor(HGF). Conclusion Tumor associated macrophages promote the migration of HCC cells by upregulating the expression of c-Met in HCC cells.

Key words: Tumor associated macrophages, Cellular-mesenchymal to epithelial transition factor, Hepatocellular carcinoma cells, Hepatocyte growth factor

CLC Number: 

  • R574
[1] Ferlay J, Shin HR, Bray F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008[J]. Int J Cancer, 2010, 127(12): 2893-2917.
[2] Forner A, Llovet JM, Bruix J. Hepatocellular carcinoma[J]. The Lancet, 2012, 379(9822): 1245-1255.
[3] 缪俊俊, 李国利, 贾筱琴. 肿瘤相关巨噬细胞在肝癌中的研究进展[J]. 肿瘤预防与治疗, 2014, 27(4): 204-208. MIAO Junjun, LI Guoli, JIA Xiaoqin. The process of tumor-associated macrophages in hepatocellular carcinoma[J]. Journal of Cancer Control and Treatment, 2014, 27(4): 204-208.
[4] Sachdeva M, Chawla YK, Arora SK, Immunology of hepatocellular carcinoma[J]. World J Hepatol, 2015, 7(17): 2080-2090.
[5] Wang H, Wang X, Li X, et al. CD68+ HLA-DR+ M1-like macrophages promote motility of HCC cells via NF-kappaB/FAK pathway[J]. Cancer Lett, 2014, 345(1): 91-99.
[6] 舒孟军, 郭圣荣. c-Met信号通路及其抑制剂的研究进展[J]. 上海交通大学学报(医学版), 2013, 33(8): 1167-1170. SHU Mengjun, GUO Shengrong. Research progress of c-Met signaling pathway and antagonists[J]. Journal of Shanghai Jiaotong University(Medical Science), 2013, 33(8): 1167-1170.
[7] Graveel CR, Tolbert D, Vande GF, et al. MET: a critical player in tumorigenesis and therapeutic target[J]. Cold Spring Harb Perspect Biol, 2013, 5(7): 1-17.
[8] Xie B, Xing R, Chen P, et al. Down-regulation of c-Met expression inhibits human HCC cells growth and invasion by RNA interference[J]. J Surg Res, 2010, 162(2): 231-238.
[9] Davis MJ, Tsang TM, Qiu Y, et al. Macrophage M1/M2 polarization dynamically adapts to changes in cytokine icroenvironments in Cryptococcus neoformans infection[J]. MBio, 2013, 4(3): e00264-13. doi:10.1128/mBio.00264-13.
[10] 周宪宾, 姚成芳. 巨噬细胞M1/M2极化分型的研究进展[J]. 中国免疫学杂志, 2012, 28(10): 957-960.
[11] Galli SJ, Borregaard N, Wynn TA. Phenotypic and functional plasticity of cells of innate immunity: macrophages, mast cells and neutrophils[J]. Nat Immunol, 2011, 12(11): 1035-1044.
[12] Murray PJ, Allen JE, Biswas SK, et al. Macrophage activation and polarization: nomenclature and experimental guidelines[J]. Immunity, 2014, 41(1): 14-20.
[13] Gardner FP, Serie DJ, Salomao DR, et al. c-MET expression in primary and liver metastases in uveal melanoma[J]. Melanoma Res, 2014, 24(6): 617-620.
[14] Balan M, Miery TE, Waaga-Gasser AM, et al. Novel roles of c-Met in the survival of renal cancer cells through the regulation of HO-1 and PD-L1 expression[J]. J Biol Chem, 2015, 290(13): 8110-8120.
[15] Surriga O, Rajasekhar VK, Ambrosini G, et al. Crizotinib, a c-Met inhibitor, prevents metastasis in a metastatic uveal melanoma model[J]. Mol Cancer Ther, 2013, 12(12): 2817-2286.
[16] Moran-Jones K, Brown LM, Samimi G. INC280, an orally available small molecule inhibitor of c-MET, reduces migration and adhesion in ovarian cancer cell models[J]. Sci Rep, 2015, 5: 11749. doi:10.1038/screp11749.
[17] Tsai PC, Fu YS, Chang LS, et al. Cardiotoxin III inhibits hepatocyte growth factor-induced epithelial-mesenchymal transition and suppresses invasion of mda-mb-231 cells[J]. J Biochem Mol Toxicol, 2016, 30(1): 12-21.
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