JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (S1): 14-17.

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Effects of Chaipu decoction on expression of VEGF and TGF-β1on airway remodeling in asthmatic rats

HUANG Yan1, KUANG Xin2, Xiong Hua3, LIU Xin1, LUO Xiao-qin3, LIU Bin3   

  1. 1. Department of Traditional Chinese Medicine, the First Hospital Affiliated to University of South China, Hengyang Hunan 421001, China;
    2. Department of Anesthesiology, the First Hospital Affiliated to University of South China, Hengyang Hunan 421001, China;
    3. University of South China, Hengyang Hunan 421001, China
  • Received:2014-11-30 Published:2014-12-30
  • Contact: 旷昕, gdl0422@qq.com E-mail:gdl0422@qq.com

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Abstract: Objective To investigate the machnism of Chaipu decoction in treatment of asthma and its effects on expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). Methods Forty SD rats randomly divided into four groups: the control group (group A), the dexamethasone group (group B), the ChaiPu decoction group (group C), the asthma chronic model group (group D). Airway remodeling was observed by H&E staining. Expression of VEGF, TGF-β1 mRNA and protein were measured by RT-PCR and immune histochemical method, respectively. Results Inflammatory cell infiltration, angiogenesis, thickening of the epithelial basement membrane and bronchial wall and bronchial narrowing were observed in D model group. The results of RT-PCR semi-quantitative analysis showed that gray value of VEGF, TGF-β1 of group D were significantly higher than other groups(P<0.05). The observed value of integral optical density (IOD) of VEGF, TGF-β1 were highest in group D (P<0.01) and lowest in group A (P<0.05 or P<0.01). Conclusion The increased expression of VEGF and TGF-β1 in asthmatic rats suggest that VEGF and TGF-β1 may be involved in airway modulating. Early use of Chaipu decoction in the treatment of asthma may down-regulate VEGF, TGF-β1 expression, therefore inhibit airway remodeling.

Key words: Chaipu decoction, VEGF, TGF-β1, Airway remodeling, Asthma

CLC Number: 

  • R361
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