JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (8): 1-5.doi: 10.6040/j.issn.1671-7554.0.2013.684

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Mechanism of human umbilical cord mesenchymal stem cells suppressing cholangiocarcinoma cell growth

LIU Juan, HAN Guoqing, LIU Hui, QIN Chengyong   

  1. Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2013-11-14 Revised:2014-07-02 Online:2014-08-10 Published:2014-08-10

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Abstract: Objective To explore the effect of human umbilical cord mesenchymal stem cells on cholangiocarcinoma HCCC-9810 cells and its molecular mechanism. Methods HCCC-9810 cells were treated with conditioned media from umbilical cord mesenchymal stem cells. Cell proliferation and apoptosis were detected with co-culture systems, MTT assay and DNA fragmentation assay; the protein expressions were determined with Western blotting and immunoflorescence staining. Results Conditioned media from hUC-MSCs could inhibit proliferation and induce apoptosis of tumor cells in a dose- and time-dependent manner. The proliferation inhibition rate increased from 12.87% to 50.98%, whereas the apoptosis rate increased from 10.1% to 49.67%. Immunoblot analysis showed that the expressions of p-Akt and p-GSK-3β were down-regulated. Conclusion The hUC-MSCs conditioned media may suppress the growth of HCCC-9810 human cholangiocarcinoma cell line via PI3K/Akt pathway.

Key words: Akt pathway, Umbilical cord mesenchymal stem cells, Cholangiocarcinoma

CLC Number: 

  • R735.7
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