Abstract:

Objective   To construct a DEC1 eukaryotic expression vector and to observe the effects of the DEC1 gene on proliferation of MGC-803 cells (a gastric cancer cell line).  Method   The DEC1 gene was amplified by RT-PCR and cloned into pGEM-T, and then into the eukaryotic expression vector pcDNA3 resulting in a recombinant vector,named pcDNA3-DEC1.pcDNA3-DEC1 was transfected into MGC-803 cells. Expression of DEC1 was detected by RT-PCR and Western blot. The effect of DEC1 on cell proliferation was measured by MTT assay and immunocytochemistry. Results   After transfection of MGC-803 cells,their DEC1 expression at both the transcript and protein levels was significantly increased, corresponding to high rates of cell proliferation. Conclusion   Expression of the DEC1 gene can promote cell proliferation of gastric cancer cells.

Key words: Gastric neoplasm ; Plasmid construction; Reverse transcription polymerase chain reaction; Transfection; Cell proliferation