Abstract:

Objective   To investigate the significance of cyclinD1 expression in ovarian cancer transplanted into nude mice. Methods   Human 3AO epithelial ovarian cancer subcutaneously transplanted models in nude mice were established. Expressions of cyclinD1 in normal tissues and ovarian cancer tissues were detected by immunochemistry and flow cytometry.  Results   Transplanted tumor models of ovarian cancer were successfully established in nude mice , and the mean success rate was 91.7%. CyclinD1 expression was negative in normal tissues. Overexpressions of cyclinD1 were detected in ovarian cancer tissues， and the positive rates were 35.00%, 55.60% and 73.30%. The apoptotic rates were 7.97%, 3.45% and 1.83%. Mean numbers of cyclinD1 were 23.15, 37.31 and 63.28. Conclusion   CyclinD1 up-regulation can be detected in ovarian cancer tissues. There is a positive correlation between the proliferation of tumor cells and expression of cyclinD1. CyclinD1 expression is related to the ovarian carcinoma development.

Key words: Epithelial ovarian neoplasms； CyclinD1； Mice, nude；  Immunochemistry; Flow Cytometry