Abstract:

Objective   To investigate the effect of a small Smac mimic molecule(compound 3) on cisplatin-induced cell apoptosis and growth inhibition of ovarian cancer cell lines. Methods   Cell viability and the growth inhibition ratio of the ovarian cancer cell lines SKOV3 were measured by MTT in the compound 3-treated group, the cisplatin-treated group and the combination treatment group. Apoptosis and protein expression were detected by flow cytometry and Western blot. Results   Cell viability was inhibited in the three groups, and it was time-dependent. The growth inhibition ratio of SKOV3 in the combination treatment group was more significantly increased than the cisplatintreated group, and the apoptotic rate of SKOV3 in the combination treatment group was more significantly increased than in the other groups. Expressions of XIAP in SKOV3 cells were decreased in the compound 3-treated group and the combination treatment group. Activated fragments of caspase 3 in the combination treatment group were more increased than in the other groups. Conclusion    Treatment with compound 3 enhances growth inhibition and apoptosis of SKOV3 induced by cisplatin. The proapoptotic effect of compound 3 was correlated with expression of XIAP.

Key words: Ovarian cancer; Smac mimic molecule; SKOV3 cell; Apoptosis; Cisplatin