JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (6): 46-50.

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Effect of xinmailong injection on proliferation and differentiation of
neural stem cells in the hippocampus of newborn rats

MAN Xiao-xiao1, YU Jun2, WANG Xiao-yun1, LIU Zhen1, LUO Shan-shan1,
XIE Zhao-hong1, ZHAO Cui-ping1， BI Jian-zhong1   

  1. 1. Department of Neurology, The Second Hospital of Shandong University, Jinan 250033, China;
    2. Department of Nosocomial Infection, Affiliated Hospital of Shandong University of
    Traditional Chinese Medicine, Jinan 250011, China
  • Received:2012-01-12 Online:2012-06-10 Published:2012-06-10

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Abstract:

Objective   To investigate the effects of xinmailong injection on the proliferation and differentiation of neural stem cells from the hippocampus of neonatal Sprague Dawley rats.   Methods   The hippocampal tissues of the newborn rat were dissociated and cultured with serum-free medium using single-cell cloning technique. Hippocampal neural stem cells of the 3rd passage were induced by xinmailong injection with various concentrations of serum and divided into the various experimental groups and the control group. Nestin, 5-bromo-2-deoxyuricine(BrdU), microtubule-associated protein 2(MAP-2), and glial fibrillary acidicprotein (GFAP) expression were detected using immunofluorescence cytochemical staining. The percentages of MAP-2 positive cells were obtained. Monotetrazolium (MTT) assay was used to detect neural stem cell (NSCs) proliferation under the condition of xinmailong injection.  Results   The percentages of MAP-2 positive cells in the various experimental groups were higher(P<0.05) than the control group. After 5 days cultivation, the NSCs growth tendency in the experimental groups was increased compared to the control group.  Conclusion   Xinmailong injection may facilitate the proliferation of NSCs and promote the differentiation of NSCs into neurons.

Key words: Nucleosides; Stem cells; Cell differentiation; Hippocampus

CLC Number: 

  • R741
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