JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (1): 34-.

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Expression of recombinant HBV in the NHMC cells and its influence on cell apoptosis

HONG Sen1，  WANG Yi-guo2，   LIU Chang-hong2，   ZHANG Peng-ju3，  LIU Qian2   

  1. 1. Shandong Academy of Medical Sciences, School of Medical and Life Sciences,   Jinan University, Jinan 250012,China;
    2. Qian Fo Shan Hospital, Shandong University, Jinan 250014, China;
    3. Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China
  • Received:2011-09-15 Online:2012-01-10 Published:2012-01-10

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Abstract:

Objective   To study the expression of a recombinant HBV in human glomerular mesangical cells (NHMC) and its influence on cell apoptosis. Methods    Recombinant full length C genotype hepatitis B virus (PHY106-CHBV) was transfected into NHMC cells by the liposome method. The supernatant of NHMC cell cultures was collected at 24， 48 and 72h after transfection. HBsAg and HBeAg were detected by the ELISA method. Morphological changes were detected by light and fluorescence microscopes after staining  with Hoechest 33258; The proliferation rate and apoptosis rate of NHMC cells were examined using the MTT assay and flow cytometry, separately. Results   The high expression levels of HBsAg and HBeAg were detected in NHMC cells after PHY106-CHBV transfection.The observed morphological changes indicated that NHMC cells′ apoptosis was induced by PHY106-CHBV. Proliferation of NHMC cells was inhibited, and apoptotic rate was elevated as shown with flow cytometry. Conclusion   HBV expression in NHMC cells led to cell proliferation arrest and promoted apoptosis.

Key words:  Hepatitis B virus; Human glomerular mesangical cell; Apoptosis

CLC Number: 

  • R512.62
[1] YANG Fang, WANG Lei. Telbivudine-resistant mutation in patients with chronic hepatitis B:  a retrospective study of 15 cases [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 129-132.
[2] SUN Haiping, WANG Lei, YANG Fang. Pyrosequencing of nucleoside (acid) analogues-resistance mutation of the P gene of hepatitis B virus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 85-.
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