Abstract:

Objective   To  investigate expressions of hypoxia- inducible factor-1α(HIF-1α)  and peroxisome proliferator-activated receptor-gamma(PPAR-γ) in traumatic kidneys, and the mechanisms of kidney trauma and repair. Methods   Rats were stricken at the skin of the renal zone by a free-fall from 45cm height to establish the traumatic rat model. Then, rats were randomly divided into 5 groups: the non-traumatic control group, and 1h, 6h, 12h and 24h post-trauma groups. Expressions of HIF-1α and PPAR-γwere detected with immunohistochemical staining. Results   Positive expression of HIF-1αwas increased in 1h and 24h post-trauma groups, located in cortex distal convoluted tubules, tubules near renal calyces and medullary tubules; while it was distinctly decreased in 6h and 12h post-trauma groups, located in tubules near renal calyces and tubules at the outer zone of renal medulla. There were significant differences in expression of HIF-1αamong all the groups(P<0.05). The PPAR-γ expression was positive in tubules of renal medulla in 1h and 24h post-trauma groups(P<0.05), however, negative in 6h and 12h post-trauma groups. Conclusion   HIF-1αand PPAR-γ may participate in the secondary hypoxia, ischemia, regeneration and repair procedures after renal injury.

Key words: Kidney; Trauma; Hypoxiainducible factor 1α; Peroxisome proliferator-activated receptor-gamma; Rats, wistar