JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• Articles • Previous Articles     Next Articles

Neuroprotective effects of Neprilysin on neuronal apoptosis induced by Amyloid-beta peptide

ZHANG Ya-lun, DING Yan, SHAO Shi-bin, YANG Ling-ling, REN Kai， DUAN Shan, ZENG Ji-ping, CUI Xing   

  1. Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China
  • Received:2007-04-26 Revised:1900-01-01 Online:2008-01-16 Published:2008-01-16
  • Contact: CUI Xing

PDF (PC)

481

Abstract: To explore the protective effects of transfection of the Neprilysin gene on primary cells against apoptosis induced by Aβ25-35. MethodsPrimary cortical neurons of fetal Wistar rats in vitro were cultured and the apoptosis cell model was induced by Aβ25-35. The NEP genes were transiently transfected into neurons. Changes of cell apoptosis were determined by MTT assay and flow cytometric DNA analysis. The expression of target mRNA and proteins was determined by RT-PCR and Western blot. ResultsExposure of primary neurons to Aβ25-35 resulted in changes of cell apoptosis. The MTT assay showed that cell activity remarkably decreased (P＜0.01) and cytometric DNA analysis showed an increase in the apoptosis rate. While in the neurons transfected with NEP genes, there were no such remarkable changes when treated with Aβ25-35. The suppression effect of the NEP gene on APP expression was shown by RT-PCR and Western blot, and the expression of Bax was decreased while that of Bcl-xl was increased after transfection. ConclusionTransfection of the NEP gene can protect primary neurons from apoptosis induced by Aβ25-35.

Key words: Neurons, Alzheimer Disease, primary culture, Neprilysin/Neutral endopeptidase

CLC Number: 

  • Q789
[1] ZHANG Dao-Lai, SUN Tao, XIE Shan-Shan, WANG Yu-Zhuo, ZHAO Ling, FENG Yu-Xin, XIN Hua. Developmental expression of NMDAR1 subunit on cultured cerebral cortical neurons of fetal rats in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 28-32.
[2] YAN Peng, WANG Rong, DU Yi-Feng, SHEN Lun-Qian. AD7c-NTP level in urine of patients with Alzheimer′s disease [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 106-.
[3] LI Guohua, CHI Heng, XING Huimin, TANG Jiyou. Effects and potential mechanism of orexin-A on hippocampal neurons under hypoxia condition [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 17-24.
[4] YUE Qingwei, ZHU Dexiao, WU Jintao, LIU Haili, ZHANG Jing, LI Guibao, DING Zhaoxi, SUN Jinhao . Methamphetamine inhibits spontaneous action potential frequency and hyperpolarization-activated cation current in mesolimbic dopaminergic neurons [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 10-14.
[5] LUO Jiangang, SUN Tao, LIN Xiaowen, LI Yun, ZHAO Fei, MIAO Guishen, FU Zhijian. Lentivirus mediated RNA interference inhibits NF-κB p65 gene expression in spinal rat cord neurons [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(3): 23-26.
[6] LI Yan-ju, TAN Shu-hui, XIA Chun-feng, ZHANG Yong, DU Yi-feng, MA Guo-zhao. Effects of the nuclear transcription factor-κB on the expression of Kir6.2 and SUR1 subunits of KATP induced by Aβ1-42 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 5-9.
[7] YIN Qing-qing, LIU Xue-ping, DONG Chuan-fang, DONG Xue-li, LI Yan-ju, LUO Ding-zhen, HOU Xun-yao. Injury effects of advanced glycation end products on the cultured
primary rat basal forebrain cholinergic neurons [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(8): 20-.
[8] ZANG Yuan-wei, GAO Lu, WU Dong, ZHANG Hong-yuan, CHEN Kai, FU Shan-shan, LIU Lu, LI Jing-xin. Tissue digestion method for isolation and characterization of
primary cultures of rat vas deferens smooth muscle cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(8): 51-56.
[9] DONG Hai-man1, GAO Qing1, ZHANG Jing1, BAO Li-hua1, LI Gui-bao1, PENG Lei1, FENG Xiao-wen1, YUE Qing-wei1, LIU Zeng-xun2, SUN Jin-hao1, GAO . Extraction of mesocortical dopaminergic projection neurons and effects of paliperidone on their electrophysiological properties [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(7): 14-18.
[10] LUO Shan-shan1, YU Jun2, WANG Xiao-yun1, LIU Zhen1, MAN Xiao-xiao1, XIE Zhao-hong1, BI Jian-zhong1. Differentiation of rat bone marrow mesenchymal stem cells into neuron like cells induced by Xinmailong injection [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(7): 32-36.
[11] ZHOU Hai-yan, LI Bao-min, YIN Ping, SUN Ruo-peng. Effect of triptolide on protection of neurons in the hippocampus of
 epileptic rats induced by lithium chloridepilocarpine [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(5): 20-.
[12] CHEN Min, SHAN Pei-yan, LIU Ai-fen, MA Lin, YU Xiao-lin. Plasma fasting glucose, fasting insulin and insulin degrading
enzyme levels in Alzheimer′s disease [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(3): 75-.
[13] YAO Yun-yi1, SUN Li2, XUE Jian-hong3, HOU Qian4, LIU Xiao-qun5, ZHOU Xiao-li5, YAN Hong-ying6, HONG Hua-rong7, YU Guo-qiang8, CHEN Fang1. Pathologic study on improvement of Alzheimer’s disease mice
treated with ethanol soluble extract of polygala [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(11): 11-.
[14] ZHU Jun-de1,2, WANG Gui-xue2, YU Yan1, YU Zi-jiang1， XIAO Zhao-lun1， WANG Yu-lin1 . Effects of basic fibroblast growth factor on cortex of parietal lobe and
middle cerebral artery in atherosclerotic model rats  [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(11): 23-.
[15] HOU Wen-xia, MENG Qing-hui, WU Jing-lin, LIU Min, GUO He. Effect of chitosan phosphatidyl choline on PKC-δ
and Iba1 in AD rats brain [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(10): 37-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd