JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Aberrant methylation of the RASSF1A gene in tissues and serums of ovarian cancer patients and its clinical significance

LI Qi-rong1，LIU Pei-shu1，ZHANG Yan2   

  1. Department of Gynecology and Obstetrics, 1. Qilu Hospital of Shandong University, Jinan 250012, Shandong, China; 2. Weifang People′s Hospital, Weifang 261041
  • Received:2007-03-09 Revised:1900-01-01 Online:2007-10-24 Published:2007-10-24
  • Contact: LIU Pei-shu

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Abstract: Objective: To explore the correlation between hypermethylation of RASSF1A gene and ovarian cancer. Method: Sixty-seven tissue samples and their corresponding serum samples from ovarian cancer patients were collected for hypermethylation measurement by semi-nested methylation-specific PCR(MSP). Results: Hypermethylation of the RASSF1A was presented in 28.4%(19/67) of the cancer serum samples and 40.3%(27/67) of the cancer tissues. None of benign ovarian disease tissues and serums was methylated. Hypermethylation of the RASSF1A was not found in serum samples from healthy volunteers. There was statistical association between hypermethylation of RASSF1A in serums and in cancer tissues (P＜0.01). Hypermethylation of the RASSF1A in serum and the corresponding cancer tissues were not significantly correlated with the clinical stage, histologic cell types, degree of cell differentiation of the tumor(P＞0.05); but it was strongly correlated with the lymph node metastasis(P＜0.01). Conclusion: Hypermethylation of the RASSF1A may be a useful marker in the auxiliary diagnosis of ovarian cancer .

Key words: Ovarian tumor, Gene, RASSF1A, DNA methylation

CLC Number: 

  • R737.31
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