JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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WANG Xu-ping, ZHAO Ling,FENG Yu-xin，SHANG Lin-shan, LIU Jin-cheng,   

  1. Institute of Cell Biology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2006-04-29 Revised:1900-01-01 Online:2006-06-24 Published:2006-06-24
  • Contact: WANG Xu-ping

Abstract: To explore the neuroprotective effects of gypenoside(GP) on glutamateinduced cytotoxicity to cortical neurons and investigate its signaling pathway. Methods: The oxidative neurotoxicity model was constructed in primary cortical neurons isolated from Wistar rat E13～14 embryos following a high dose glutamate to the neurons directly. GP was added before the damage. The viability of cells after various treatments was detected by MTT method. Ho33342 and PI double labeled method was used to analyse cellular death pathways. The contents of NO and H2O2 were detected. The changes of mitochondria membrane potential of neurons were analyzed by flow cytometry.Results: Glutamate triggered two cellular death pathways:

Key words: Gynostemma, Glutamic acid, Neurons, Oxidative neurotoxicity

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