Journal of Shandong University (Health Sciences) ›› 2024, Vol. 62 ›› Issue (5): 64-71.doi: 10.6040/j.issn.1671-7554.0.2023.0950

• Preclinical Medicine • Previous Articles    

Therapeutic effects of aconitum carmichaelii decoction on a rat model of knee osteoarthritis

WANG Lisha1, WANG Shangzeng2, SHI Dongliang1, ZHANG Zhongbo1, REN Bowen1, WANG Yunfei1, GUO Zhonghua1, ZHOU Xiaoning3   

  1. 1. Department of Osteopathy, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou 450053, Henan, China;
    2. Department of Arthrosis, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou 450053, Henan, China;
    3. Department of Neck, Shoulder, Waist and Leg Pain, Luoyang Orthopedic Hospital, Luoyang 450016, Henan, China
  • Published:2024-05-29

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Abstract: Objective To investigate the therapeutic effects of aconitum carmichaelii decoction based on the nuclear factor E2 related factor 2(Nrf2)/heme oxygenase-1(HO-1)pathway on a rat model of knee osteoarthritis(KOA). Methods KOA rat model was established by injecting papain solution into the knee joint; the rats after modeling were randomly grouped into KOA group, aconitum carmichaelii decoction group(4.2 g/kg aconitum carmichaelii decoction), inhibitor group(30 mg/kg ML385), aconitum carmichaelii decoction+inhibitor group(4.2 g/kg aconitum carmichaelii decoction+30 mg/kg ML385), with 10 rats in each group, and another 10 rats were injected intracavitally with physiological saline as the control group. Hematoxylin-eosin staining was used to observe the histopathological changes of cartilage in cartilage and to score the damage; serum indicators of inflammatory factor ［tumor necrosis factor-α(TNF-α)/interleukin-1β(IL-1β)］ and oxidative stress ［malondialdehyde(MDA)and superoxide dismutase(SOD)］ were measured by ELISA; Nrf2 and HO-1 expressions were detected by qRT-PCR and Western blotting. Results Compared with the control group, the cartilage surface of KOA group was rough and uneven, disordered cell arrangement, and disappearance of lamellar structure, increased content of IL-1β, TNF-α and MDA, and decreased expressions of SOD, Nrf2, and HO-1 mRNA and protein(P<0.05); compared with the KOA group, the pathological damage of aconitum carmichaelii decoction group was relieved, with decreased contents of IL-1β, TNF-α, and MDA, and increased expressions of SOD, Nrf2, and HO-1 mRNA and protein(P<0.05); however, the pathological damage in the inhibitor group was further aggravated, with increased content of IL-1β, TNF-α, and MDA, and decreased expressions of SOD, Nrf2, and HO-1 mRNA and protein(P<0.05); compared with the aconitum carmichaelii decoction group, aconitum carmichaelii decoction+inhibitor group had more serious pathological damage, with increased content of IL-1β, TNF-α, and MDA, and decreased expressions of SOD, Nrf2, and HO-1 mRNA and protein(P<0.05); compared with the inhibitor group, the pathological damage in the aconitum carmichaelii decoction+inhibitor group was slightly improved, with decreased content of IL-1β, TNF-α, and MDA, and increased expressions of SOD, Nrf2, and HO-1 mRNA and protein(P<0.05). Conclusion Aconitum carmichaelii decoction can treat KOA rats by activating Nrf2/HO-1 pathway.

Key words: Aconitum carmichaelii decoction, Nuclear factor E2 related factor 2/Heme oxygenase-1 pathway, Knee osteoarthritis, Therapeutic mechanism

CLC Number: 

  • R274
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