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山东大学学报 (医学版) ›› 2017, Vol. 55 ›› Issue (12): 1-6.doi: 10.6040/j.issn.1671-7554.0.2016.1702

• 基础医学 •    下一篇

EGCG抑制内质网应激减轻高糖致HK-2细胞凋亡的作用

项春红1,吕丽2*,江蓓1,肖晓燕1,胡昭1   

  1. 1.山东大学齐鲁医院肾内科, 山东 济南 250012;2.内蒙古科技大学包头医学院第一附属医院肾内科, 内蒙古 包头 014010
  • 出版日期:2017-12-20 发布日期:2022-09-27
  • 通讯作者: 胡昭. E-mail:sdhuzhao@163.com*共同第一作者.
  • 基金资助:
    中国博士后科学基金面上项目(2015M572048);齐鲁医院临床研究项目(2014QLKY13)

Mechanism of EGCG protecting high-glucose-induced HK-2 cell apoptosis by suppressing endoplasmic reticulum stress

XIANG Chunhong1, LÜ Li2*, JIANG Bei1, XIAO Xiaoyan1, HU Zhao1   

  1. 1. Department of Nephrology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Nephrology, First Affiliated Hospital of Baotou Medical College of Inner Mongolia, Baotou 014010, Inner Mongolia, China
  • Online:2017-12-20 Published:2022-09-27

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摘要： 目的 探究表没食子儿茶素没食子酸酯(EGCG)对高糖环境下人肾小管上皮细胞(HK-2)的保护作用。 方法 根据高糖可诱导HK-2细胞损伤将细胞分为正常糖组、甘露醇组、高糖组及不同浓度的EGCG组,干预HK-2细胞24 h后,采用CCK8法检测HK-2细胞的增殖。Hoechst33258染色和Annexin V法检测正常糖组、甘露醇组、高糖组、(20 μmol/L)EGCG组作用24 h后HK-2细胞凋亡;Western blotting检测上述4组HK-2细胞内质网应激反应蛋白GRP78和Caspase-12蛋白的表达。 结果 高糖组培养HK-2 细胞24 h后,细胞增殖较正常糖组明显降低,细胞凋亡较正常糖组明显增加(P<0.001)。(20 μmol/L)EGCG组干预24 h后能明显改善高糖环境下HK-2细胞的增殖。EGCG(20 μmol/L)作用24 h后能明显降低细胞的凋亡率(P<0.001),且EGCG干预后内质网应激反应蛋白GRP78和Caspase-12蛋白表达较高糖组也明显降低(P=0.001)。 结论 EGCG可能通过抑制内质网应激介导的凋亡途径改善高糖致HK-2细胞的凋亡。

关键词: 表没食子儿茶素没食子酸酯, 内质网应激, 人肾小管上皮细胞, 细胞凋亡

Abstract: Objective To investigate the protective effect and mechanism of epigallocatechin 3-gallate(EGCG)on HK-2 cells exposed to high glucose. Methods The HK-2 cells were divided into normal glucose group, mannitol group, high glucose group, and EGCG group. After 24-hour treatment, cell proliferation was measured with CCK8; cell apoptosis was measured with Hoechst33258 and Annexin V staining; the expressions of GRP78 and Caspase-12 were determined with Western blotting. Results Compared with the normal glucose group, the high glucose group showed significantly inhibited cell proliferation and increased apoptosis(P<0.001), while the EGCG(20 μmol/L)group displayed significantly improved cell proliferation, reduced apoptosis rate(P<0.001), and decreased GRP78 and Caspase-12 expressions(P=0.001). Conclusion EGCG may reduce high-glucose-induced HK-2 apoptosis by inhibiting endoplasmic reticulum stress.

Key words: Epigallocatechin 3-gallate, Endoplasmic reticulum stress, HK-2 cells, Apoptosis

中图分类号: 

  • R692.6
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