磷脂酰肌醇-4-磷酸酶II型对宫颈癌细胞增殖及凋亡的影响

doi:10.6040/j.issn.1671-7554.0.2016.1425

摘要/Abstract

摘要： 目的探讨磷脂酰肌醇-4-磷酸酶II型(INPP4B)对宫颈癌细胞生长增殖、细胞周期及凋亡的影响。方法 Western blotting检测常见的宫颈癌细胞系中INPP4B的基础表达水平,对INPP4B阴性的SiHa、C33a细胞转染表达质粒LHCX-INPP4B,高表达INPP4B的CaSki细胞转染INPP4B特异性的siRNA,Western blotting检测细胞中INPP4B表达的变化。CCK-8、克隆形成实验检测INPP4B对宫颈癌细胞生长增殖的影响;BrdU染色检测细胞周期,Annexin V-FITC/PI双染后流式细胞仪检测细胞凋亡。结果 CCK-8和克隆形成实验显示,过表达INPP4B使SiHa和C33a细胞的活性和克隆形成能力明显下降(P均<0.05); BrdU实验表明,转染INPP4B后SiHa细胞和C33a细胞进入S期的比例减少(P=0.04,P=0.01)。与之相应,干扰INPP4B表达后CaSki细胞的生长增殖能力增强,进入S期的细胞比例增加(P<0.01)。但INPP4B表达的变化对SiHa、C33a和CaSki细胞的凋亡无明显影响。结论 INPP4B可抑制宫颈癌细胞的生长增殖和细胞周期进程。

关键词: 宫颈癌, 磷脂酰肌醇-4-磷酸酶II型, 周期, 增殖

Abstract: Objective To study the effect of inositol polyphosphate-4-phosphatase type II(INPP4B)on the proliferation,cell cycle and apoptosis of cervical cancer cells. Methods Western blotting was employed to detect basic expression of INPP4B in various kinds of cervical cancer cell lines. The INPP4B expressions in LHCX-INPP4B transfected SiHa and C33a cells as well as INPP4B specific siRNA transfected CaSki cells were determined by Western blotting. CCK-8 and colony formation assay were used to test cell growth and proliferation. Cell cycle was detected by using BrdU assay. Cell apoptosis was evaluated using Annexin V-FITC/PI cell apoptosis kit. Results CCK-8 and clone formation assay showed that the overexpression of INPP4B significantly reduced the cell viability and clonogenic ability of SiHa and C33a(all P<0.05). BrdU assay showed that the proportion of SiHa and C33a cells in S phase decreased after being transfected with INPP4B(P=0.04, P=0.01). Accordantly, depletion of INPP4B in Caski cells increased cell viability and the percentage of the cells entering S phase(P<0.01). However, INPP4B did not influence the apoptosis of cervical cancer cells. Conclusion INPP4B inhibits the growth and proliferation of cervical cancer cells and leads to cell cycle arrest.

Key words: Inositol polyphosphate-4-phosphatase type II, Cycle, Cervical cancer, Proliferation

中图分类号:

R737

孙泽雨,陈颖,林家香,刘娟,赵蔚明. 磷脂酰肌醇-4-磷酸酶II型对宫颈癌细胞增殖及凋亡的影响[J]. 山东大学学报（医学版）, 2017, 55(11): 1-6.

SUN Zeyu, CHEN Ying, LIN Jiaxiang, LIU Juan, ZHAO Weiming. Influence of inositol polyphosphate-4-phosphatase type II on the proliferation and apoptosis of cervical cancer cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 1-6.

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