山东大学学报(医学版) ›› 2017, Vol. 55 ›› Issue (1): 69-74.doi: 10.6040/j.issn.1671-7554.0.2016.1313
李婧博,刘洪彬,贾月月,王泽,孙梅,石玉华
LI Jingbo, LIU Hongbin, JIA Yueyue, WANG Ze, SUN Mei, SHI Yuhua
摘要: 目的 检测多囊卵巢综合征(PCOS)胰岛素抵抗患者卵巢颗粒细胞中microRNA-183(miR-183)的表达,并评价其临床意义。 方法 采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测120例PCOS患者[其中 78例PCOS胰岛素抵抗患者(PCOS胰岛素抵抗组)和42例PCOS非胰岛素抵抗患者(PCOS非胰岛素抵抗组)及72例正常对照者(正常对照组)]颗粒细胞中miR-183的表达,并对miR-183表达和胰岛素抵抗指数(HOMA-IR)进行关联分析,同时对组间临床资料进行回顾性分析。采用受试者工作特征(ROC)曲线评价miR-183的诊断效能。 结果 miR-183在PCOS胰岛素抵抗组颗粒细胞中的表达高于正常对照组(P<0.001)及非胰岛素抵抗组(P=0.034),PCOS胰岛素抵抗组miR-183的表达与HOMA-IR呈正相关(r=0.476, P=0.003),ROC曲线提示miR-183联合体质量指数(BMI)作为PCOS胰岛素抵抗的评价指标,其诊断效能较高(AUC: 0.820, 95%CI: 0.750~0.889, P<0.001)。 结论 miR-183在PCOS胰岛素抵抗患者颗粒细胞中高表达,并与HOMA-IR相关,其可能通过卵泡局部微环境参与调控PCOS胰岛素抵抗的致病过程。
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