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山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (11): 24-26.doi: 10.6040/j.issn.1671-7554.0.2015.542

• 基础医学 • 上一篇    下一篇

炎性细胞模型中姜黄素对胆固醇逆转运蛋白ABCA1和ABCG1基因的影响

吴彧1*,夏彦清2*,伍玥2,孙琳1,邬成业2,娄桂予2,李雅欣2,丁松泽2,孙恺2   

  1. 河南省人民医院 1.康馨心内科;2.中心实验室, 河南 郑州 450003
  • 收稿日期:2015-06-04 出版日期:2016-11-10 发布日期:2016-11-10
  • 通讯作者: 孙琳. E-mail:zsunlin@sina.com 孙恺. E-mail:ssskkk1112000@163.com*共同第一作者. E-mail:zsunlin@sina.com
  • 基金资助:
    郑州市金水区科技攻关项目(金科［2012］23号);河南省医学科技攻关计划省部共建项目(201201005)

Effects of curcumin on gene expressions of reverse cholesterol transport proteins ABCA1versus ABCG1 in the cell model of inflammation

WU Yu1*, XIA Yanqing2*, WU Yue2, SUN Lin1, WU Chengye2, LOU Guiyu2, LI Yaxin2, DING Songze2, SUN Kai2   

  1. 1. Department of Kangxin Cardiology;
    2.Center for Translational Medicine, Henan Provincial Peoples Hospital, Zhengzhou 450003, Henan, China
  • Received:2015-06-04 Online:2016-11-10 Published:2016-11-10

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摘要： 目的 探讨炎性反应模型中,姜黄素对胆固醇逆转运蛋白基因表达的影响。 方法 将RAW264.7细胞分为6组:空白对照组,姜黄素10 μmol/L组,姜黄素20 μmol/L组,脂多糖(LPS)组,姜黄素10 μmol/L+LPS组和姜黄素20 μmol/L+LPS组。应用荧光定量PCR检测各组中RAW264.7细胞腺苷三磷酸结合盒转运体A1(ABCA1)、腺苷三磷酸结合盒转运体G1(ABCG1)和肿瘤坏死因子-α(TNF-α)基因表达情况。 结果 LPS刺激可显著上调RAW264.7细胞ABCA1和TNF-α基因的表达(P<0.05),下调ABCG1基因的表达(P<0.05);姜黄素处理后可进一步上调ABCA1基因的表达(P<0.05),但是对ABCG1和TNF-α基因的表达差异无统计学意义(P>0.05)。 结论 在炎性反应条件下,姜黄素可进一步上调胆固醇逆转运关键基因ABCA1的表达,这可能是其抗动脉硬化的分子机制之一。

关键词: 动脉硬化, 姜黄素, 腺苷三磷酸结合盒转运体A1, 脂多糖

Abstract: Objective To explore the effects of curcumin on reverse cholesterol transport protein gene expression in the cell model of inflammation. Methods RAW264.7 cells were divided into 6 groups: control group, 10 μmol/L curcumin group, 20 μmol/L curcumin group, lipopolysaccharide(LPS)group, 10 μmol/L curcumin+LPS group, and 20 μmol/L curcumin+LPS group. The levels of mRNA expressions of ATP-binding cassette transporter A1(ABCA1), ATP-binding cassette transporter G1(ABCG1)and tumor necrosis factor-α(TNF-α)in each group were determined by Real-Time PCR. Results LPS induction significantly increased ABCA1 and TNF-α mRNA expressions, and decreased ABCG1 mRNA expression(all P<0.05). Pre-treating the cell with curcumin and stimulating with LPS did not change the expressions of TNF-α and ABCG1(both P>0.05), but significantly increased the ABCA1 mRNA expression(P<0.05). Conclusion Curcumin significantly amplifies the expression of reverse cholesterol transport key genes ABCA1 under the condition of the inflammatory reaction, which may be one of the molecular mechanisms of anti-arteriosclerosis.

Key words: Curcumin, Lipopolysaccharide, ATP-binding cassette transporter A1, Arteriosclerosis

中图分类号: 

  • R34
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