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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (11): 16-20.doi: 10.6040/j.issn.1671-7554.0.2014.880

• 基础医学 • 上一篇    下一篇

八肽胆囊收缩素对脂多糖诱导的小鼠单核细胞RAW264.7细胞内质网应激的影响

段林1, 张晓静2, 于盼盼2, 于峰2, 刘威3, 宋宁1   

  1. 1. 河北医科大学第二医院呼吸内科, 河北 石家庄 050000;
    2. 河北医科大学基础医学院法医学系, 河北 石家庄 050017;
    3. 河北省胸科医院骨科, 河北 石家庄 050041
  • 收稿日期:2014-11-28 出版日期:2015-11-10 发布日期:2015-11-10
  • 通讯作者: 刘威.E-mail:zhangxue781127@163.com;宋宁.E-mail:342840431@qq.com E-mail:zhangxue781127@163.com;342840431@qq.com
  • 基金资助:
    河北省自然科学基金(H2012206029)

Effects of cholecystokinin-octopeptide on endoplasmic reticulum stress induced by LPS in mouse monocyte RAW264.7 cells

DUAN Lin1, ZHANG Xiaojing2, YU Panpan2, YU Feng2, LIU Wei3, SONG Ning1   

  1. 1. Department of Respiratory Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China;
    2. Department of Forensic Medicine, Basic Medical College, Hebei Medical University, Shijiazhuang 050017, Hebei, China;
    3. Department of Orthopaedics, Hebei Chest Hospital, Shijiazhuang 050041, Hebei, China
  • Received:2014-11-28 Online:2015-11-10 Published:2015-11-10

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摘要： 目的 探讨八肽胆囊收缩素(CCK-8)对脂多糖(LPS)诱导的单核细胞RAW264.7细胞内质网应激(ERS)的影响.方法 RAW264.7细胞分为对照组、LPS组、CCK-8组、devazepide组(DEV组)、CCK-8+LPS组及DEV+CCK-8+LPS组,采用RT-PCR法检测X盒结合蛋白1的活性形式(XBP1s)的mRNA表达水平,Western blotting检测ERS标志性蛋白葡萄糖调节蛋白78(GRP78)和C/EBP同源蛋白(CHOP)的蛋白表达水平,ELISA法检测细胞培养上清液中IL-1β水平.结果 与对照组相比,LPS组的XBP1s mRNA表达水平、GRP78和CHOP的蛋白表达水平均升高(P<0.05);与LPS组相比,CCK-8+LPS组的XBP1s mRNA表达水平、GRP78和CHOP的蛋白表达水平均降低(P<0.05);与CCK-8+LPS组相比,DEV+CCK-8+LPS组的XBP1smRNA和GRP78、CHOP蛋白表达水平均升高(P<0.05).IL-1β含量的变化趋势与CHOP蛋白表达的变化趋势一致.结论 CCK-8通过1受体抑制LPS诱导的ERS,减少IL-1β的生成,从而发挥抗炎作用.

关键词: 八肽胆囊收缩素, X盒结合蛋白1, 葡萄糖调节蛋白78, C/EBP同源蛋白, 脂多糖

Abstract: Objective To investigate the effects of cholecystokinin-octopeptide (CCK-8) on ER stress (ERS) induced by lipopolysaccharide (LPS) in mouse monocyte RAW264.7 cells. Methods RAW264.7 cells were divided into control group, LPS group, CCK-8 grpup, devazepide group(DEV group), CCK-8+LPS group, and DEV+CCK-8+LPS group. The active form of X-box binding protein 1(XBP1s) mRNA expression was assayed by RT-PCR, glucose regulating protein 78 (GRP78) and C/EBP homologous protein (CHOP) protein expressions were determined by Western blotting, and IL-1β expression levels were measured by ELISA. Results Compared with control group, the expressions of XBP1s mRNA and GRP78 and CHOP protein in LPS group increased (P<0.05); cmpared with LPS group, the expressions of XBP1s mRNA and GRP78 and CHOP protein in CCK-8+LPS group decreased (P<0.05); compared with CCK-8+LPS group, the expressions of XBP1s mRNA and GRP78 and CHOP protein in DEV+CCK-8+LPS group increased (P<0.05). The changes of IL-1β contents showed the same trend as that of CHOP expression (P<0.05). Conclusion CCK-8 exerts an anti-inflammatory effect by inhibiting LPS-induced ERS and decreasing the expression of IL-1β in RAW264.7 cells.

Key words: Cholecystokinin-octopeptide, Lipopolysaccharides, X-box binding protein 1, C/EBP homologous protein, Glucose regulating protein 78

中图分类号: 

  • R541.4
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