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山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (10): 25-28.doi: 10.6040/j.issn.1671-7554.0.2016.587

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糖尿病大鼠阴茎海绵体硫化氢含量及其合成酶表达的变化

赵蕙琛1,2*,柴家超3,4*,张亮5,袁明振6,彭力7,刘元涛1   

  1. 1.青岛市立医院内分泌科, 山东 青岛 266071;2.山东大学第二医院肾内科, 山东 济南 250033;3.山东大学附属省立医院小儿外科, 山东 济南 250021;4.青岛市妇女儿童医院普外科, 山东 青岛 266011;5.山东大学第二医院内分泌科, 山东 济南 250033;6.山东大学第二医院泌尿外科, 山东 济南 250033;7.济南市第四人民医院干部保健病房, 山东 济南 250031
  • 收稿日期:2016-05-23 出版日期:2016-10-10 发布日期:2016-10-10
  • 通讯作者: 刘元涛. E-mail:sduliuyuantao@163.com*共同第一作者. E-mail:sduliuyuantao@163.com
  • 基金资助:
    山东省科技发展计划(2010GWZ20248)

Changes of hydrogen sulfide concentrations and synthetase expressions in the corpus cavernosum of diabetic rats

ZHAO Huichen1,2*, CHAI Jiachao3,4*, ZHANG Liang5, YUAN Mingzhen6, PENG Li7, LIU Yuantao1   

  1. 1. Department of Endocrinology, Qingdao Municipal Hospital, Qingdao 266071, Shandong, China;
    2. Department of Nephrology, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    3. Department of Pediatric Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    4. Department of General Surgery, Qingdao Women and Childrens Hospital, Qingdao 266011, Shandong, China;
    5. Department of Endocrinology, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    6. Department of Urology, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    7. Cadres Ward, Jinan Fourth Peoples Hospital, Jinan 250031, Shandong, China
  • Received:2016-05-23 Online:2016-10-10 Published:2016-10-10

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摘要： 目的 观察糖尿病大鼠及非糖尿病大鼠阴茎海绵体组织中硫化氢(H2S)含量及胱硫醚-γ-裂解酶(CSE)表达的差异。 方法 将Wistar大鼠随机平均分为两组,一组高脂饲料喂养8周后,注射链脲佐菌素(STZ)制备2型糖尿病大鼠模型(糖尿病组),另一组普通饲料喂养(对照组)。继续喂养12周后分离两组大鼠海绵体组织,检测海绵体组织内源性H2S的生成;采用RT- PCR法检测两组海绵体组织中CSE mRNA的表达;采用Western blotting法检测两组CSE蛋白的表达。 结果 糖尿病组内源性H2S浓度较对照组显著降低(P<0.01);糖尿病组海绵体组织中CSE mRNA和CSE蛋白的表达量均较对照组显著降低(P<0.01)。 结论 糖尿病大鼠阴茎海绵体H2S含量显著降低,其机制可能与CSE mRNA及蛋白水平表达减少有关。

关键词: 大鼠, 勃起功能障碍, 硫化氢, 胱硫醚-γ-裂解酶, 糖尿病

Abstract: Objective To examine the endogenous hydrogen sulfide(H2S)concentrations and expressions of cystathionine-γ-lyase(CSE)in the corpus cavernosum tissues of diabetic and non-diabetic rats. Methods Rats were randomly divided into two groups. One group was fed with high-fat diet for 8 weeks and injected with streptozocin(STZ)to create type 2 diabetic rat models. The other group was fed with normal diet to serve as controls. All mice were killed and corpus cavernosum tissues were harvested. After that H2S concentrations in corpus cavernosum tissues were measured. The mRNA and protein expressions of CSE were detected with RT-PCR and Western blotting respectively. Results 山 东 大 学 学 报 (医 学 版)54卷10期 -赵蕙琛,等.糖尿病大鼠阴茎海绵体硫化氢含量及其合成酶表达的变化 \=-The H2S level in the corpus cavernosum tissues was significantly decreased in the diabetic group compared with the controls(P<0.01). The mRNA and protein expressions of CSE were significantly decreased in the diabetic group(both P<0.05). Conclusion H2S concentration was significantly decreased in the corpus cavernosum tissues of diabetic rats, which may be associated with the down-regulation of mRNA and protein expressions of CSE.

Key words: Diabetes, Cystathionine-γ-lyase, Erectile dysfunction, Hydrogen sulfide, Rat

中图分类号: 

  • R587.2
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