您的位置：山东大学 -> 科技期刊社 -> 《山东大学学报（医学版）》

山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (6): 50-54.doi: 10.6040/j.issn.1671-7554.0.2015.1222

• • 上一篇    下一篇

BDNF/TrkB在舌鳞状细胞癌中的表达及BDNF对其增殖能力的影响

魏秀娟1,吴修胤2, 佟冬冬1,李静1,杨晓露1,张风河1   

  1. 1. 山东大学口腔医院口腔颌面外科, 山东 济南 250012;2. 山东省莱芜市人民医院口腔科, 山东 莱芜 271100
  • 收稿日期:2015-12-03 出版日期:2016-06-20 发布日期:2016-06-20
  • 通讯作者: 张风河. E-mail:zfengh@sdu.edu.cn E-mail:zfengh@sdu.edu.cn
  • 基金资助:
    山东省科技计划(2014GGH218038)

Expression of BDNF/TrkB and effect of BDNF on the proliferation ability of tongue squamous cell carcinoma

WEI Xiujuan1, WU Xiuyin2, TONG Dongdong1, LI Jing1, YANG Xiaolu1, ZHANG Fenghe1   

  1. 1. Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Stomatology, Peoples Hospital of Laiwu City, Laiwu 271100, Shandong, China
  • Received:2015-12-03 Online:2016-06-20 Published:2016-06-20

PDF (PC)

202

摘要： 目的 研究脑源性神经营养因子(BDNF)及酪氨酸蛋白激酶受体B(TrkB)在舌鳞状细胞癌(TSCC)中的表达情况,并观察外源性BDNF对舌鳞状细胞癌增殖活性的影响。 方法 采用免疫组织化学技术检测BDNF、TrkB在50例TSCC和13例正常舌黏膜中的表达;以舌鳞癌细胞系Tca8113为研究对象,采用免疫荧光技术检测BDNF、TrkB的表达,CCK8细胞增殖实验观察外源性BDNF对舌鳞癌细胞系Tca8113增殖活性的影响。 结果 BDNF/TrkB在舌鳞状细胞癌组织标本中阳性表达率显著高于正常舌黏膜组织(P<0.05),且两者的表达与浸润深度及淋巴转移有关。高浓度BDNF(200 ng/mL)可显著促进Tca8113的增殖活性(P<0.05)。 结论 BDNF/TrkB可能与舌鳞状细胞癌的发生发展密切相关,BDNF可显著提高舌鳞状细胞癌的增殖能力。

关键词: 酪氨酸蛋白激酶受体B, 舌鳞状细胞癌, 脑源性神经营养因子, 增殖能力

Abstract: Objective To investigate the expressions of brain-derived neurotrophic factor(BDNF)and its primary receptor tyrosine kinase receptor B(TrkB)in tongue squamous cell carcinoma(TSCC), and to explore the effect of BDNF on the proliferation of TSCC. Methods The expressions of BDNF and TrkB in 50 TSCC and 13 normal mucosa tissues were detected with immunohistochemical method. The expressions of BDNF and TrkB in human tongue squamous cell carcinoma cell lines(Tca8113)were examined with immunofluorescence technique. Cell viability of Tca8113 was detected with CCK8 assay. Results The positive rate of staining of BDNF and TrkB in TSCC was significantly higher than that in normal tongue mucosa(P<0.05). In addition, the rate of positive expression was related to infiltration depth and lymph node metastasis. BDNF(200 ng/mL)significantly promoted the proliferation of Tca8113. Conclusion BDNF and TrkB may be closely associated with the genesis and development of TSCC. Moreover, BDNF may promote cell proliferation of TSCC.

Key words: Brain-derived neurotrophic factor, Tyrosine kinase receptor B, Tongue squamous cell carcinoma, Proliferation ability

中图分类号: 

  • R782
[1] Lin CY, Chen HJ, Li TM, et al. β5 Integrin up-regulation in brain-derived neurotrophic factor promotes cell motility in human chondrosarcoma[J]. PLoS One, 2013, 8(7): e67990. doi: 10.1371/journal.pone.0067990.
[2] Zhang S, Guo D, Luo W, et al. TrkB is highly expressed in NSCLC and mediated BDNF-induced the activation of Pyk2 signaling and the invasion of A549 cells[J]. BMC Cancer, 2010, 10: 43. doi: 10.1186/1471-2407-10-43.
[3] 刘建英, 李坤霞, 李爱敏, 等. TrkB-BDNF信号传导通路对神经母细胞瘤细胞耐药和转移的影响[J]. 中华小儿外科杂志, 2013, 34(8): 607-612. LIU Jianying, LI Kunxia, LI Aimin, et al. Effects of TrkB-BDNF signaling pathway on chemoresistance and metastasis of neuroblastoma cells[J]. Chin J Pediatr Surg, 2013, 34(8):607-612.
[4] Li Z, Oh DY, Nakamura K, et al. Perifosine-induced inhibition of Akt attenuates brain-derived neurotrophin factor /TrkB-induced chemoresistance in neuroblastoma in vivo[J]. Cancer, 2011, 117(23):5412-5422.
[5] 郑家伟, 李金忠, 钟来平, 等. 口腔鳞状细胞癌临床流行病学研究现状[J]. 中国口腔颌面外科杂志, 2007, 5(2):83-90. ZHENG Jiawei, LI Jinzhong, ZHONG Laiping, et al.Clinical epidemiology and risk factors of oral squamous cell carcinoma: an overview[J]. China Journal of Oral and Maxillofacial Surgery, 2007, 5(2):83-90.
[6] Ng YK, Wong EY, Lau CP, et al. K252a induces anoikissensitization with suppression of cellular migration in Epstein-Barr virus(EBV)-associated nasopharyngeal carcinoma cells[J]. Invest New Drugs, 2012, 30(1):48-58.
[7] Sclabas GM, Fujioka S, Schmidt C, et al. Overexpression of tropomysin-related kinase B in metastatic human pancreatic cancer cells[J]. Clin Cancer Res, 2005, 11(2Pt1):440-449.
[8] Odate S, Nakamura K, Onishi H, et al. TrkB/BDNF signaling pathway is a potential therapeutic target for pulmonary larger cell neuroendocrine carcinoma[J]. Lung Cancer, 2013, 79(3):205-214.
[9] Au CW, Siu MK, Liao X, et al. Tyrosine kinase B receptor and BDNF expression in ovarian cancers-Effect on cell migration, angiogenesis and clinical outcome[J]. Cancer Lett, 2009, 281(2):151-161.
[10] Bronzetti E, Artico M, Forte F, et al. A possible role of BDNF in prostate cancer detection[J]. Oncol Rep, 2008, 19(4):969-974.
[11] 刘天卿, 任海军, 温爽, 等. 胃癌组织中Survivin、TrkB和BDNF的表达及意义[J]. 临床与实验病理学杂志, 2009, 25(3):291-294. LIU Tianqing, REN Haijun, WEN Shuang, et al. Expression and significance of survivin, TrkB and BDNF in gastric carcinoma[J]. Clin Exp Pathol, 2009, 25(3):291-294.
[12] Douma S, Van Laar T, Zevenhoven J, et al. Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB[J]. Nature, 2004, 430(3):1034-1039.
[13] Yang ZF, Ho DW, Lam CT, et al. Identification of brain-derived neurotrophic factor as a novel functional protein in hepatocellular carcinoma[J]. Cancer Res, 2005, 65(1):219-225.
[14] 孙春艳, 胡豫, 郭涛, 等. 脑源性神经营养因子激活其受体TrkB在多发性骨髓瘤发病机制中的作用[J]. 中华血液学杂志, 2008, 29(4):238-242. SUN Chunyan, HU Yu, GUO Tao, et al. Study of brain-derived neurotrophic factor activating TrkB signaling cascades in the pathogenesis of multiple myeloma[J]. Clin J Hematol, 2008, 29(4):238-242.
[15] Hu Y, Sun CY, Wang HF, et al. Brain-derived neurotrophic factor promotes growth and migration of multiple myeloma cells[J]. Cancer Genet Cytoqenet, 2006, 169(1):12-20.
[16] Serres F, Carney SL. Nicotine regulates SH-SY5Y neuroblastoma cell proliferation through the release of brain-derived neurotrophic factor[J]. Brain Res, 2006, 1101(1):36-42.
[17] Miknyoczki SJ, Wan W, Chang H, et al. The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice[J]. Clin Cancer Res, 2002, 8(6):1924-1931.
[1] 张文雪,徐丽东,张明明,郭传国,左秀丽. NR2B通过mBDNF参与肠易激综合征内脏高敏感的发生[J]. 山东大学学报（医学版）, 2017, 55(7): 17-22.
[2] 陈磊,刘东晓,李开鸣,宋新强,曾宪思,蒋丽杰. 有序胶原材料联合CBD-BDNF对大鼠脊髓损伤的修复作用[J]. 山东大学学报（医学版）, 2017, 55(5): 43-48.
[3] 赵栋燕,于岩波,左秀丽. 脑源性神经营养因子在小鼠肠黏膜屏障中的作用[J]. 山东大学学报（医学版）, 2016, 54(7): 1-5.
[4] 袁秀玉,董原君,梁霞,胡敏,张桂青. 创伤性应激对大鼠海马BDNF表达的影响[J]. 山东大学学报（医学版）, 2016, 54(4): 37-41.
[5] 曹静, 陈飞雪, 王腾飞, 赵宏宇, 赵栋燕, 左秀丽, 李延青. 脑源性神经营养因子对小鼠结肠平滑肌细胞的作用及其机制[J]. 山东大学学报（医学版）, 2015, 53(12): 1-6.
[6] 赵宏宇，陈飞雪，曹静，戚庆庆，李月月，左秀丽，李延青. 脑组织海马区脑源性神经营养因子在焦虑调节内脏高敏感中的作用[J]. 山东大学学报（医学版）, 2014, 52(3): 33-36.
[7] 王晓蕾1,郏雁飞2,郑燕2,刘华2,汪运山2. STAT3真核表达质粒的构建及对胃癌细胞增殖的影响[J]. 山东大学学报(医学版), 2013, 51(9): 45-49.
[8] 陈乃耀1，申娜2，贺靖澜3，郑丽坤4. 人脐血间充质干细胞治疗鼠创伤性脑损伤后BDNF的变化[J]. 山东大学学报(医学版), 2013, 51(1): 22-.
[9] 陈飞雪，于岩波，王鹏，左秀丽，李延青. 脑源性神经营养因子对小鼠回肠及结肠平滑肌收缩活动的影响及其机制[J]. 山东大学学报(医学版), 2012, 50(4): 42-46.
[10] 刘杨，苗宇船. 滋补脾阴方药对大鼠脊髓损伤后脑源性神经营养因子表达的影响[J]. 山东大学学报(医学版), 2012, 50(10): 33-36.
[11] 张东1，何海贤2，董立新3，陈正岗4，王玉敏1，刘少华1. 舌鳞癌组织中半乳糖凝集素-1的表达及其与临床病理因素的相关性研究[J]. 山东大学学报(医学版), 2011, 49(11): 138-.
[12] 姜丽,于灵芝,刘旭东,郭兰敏 . 外源性VEGF对鼠脊髓缺血再灌注后内皮抑素和脑源性神经营养因子及其受体的影响[J]. 山东大学学报(医学版), 2008, 46(6): 586-589.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2018 《山东大学学报(医学版)》编辑部　
地址:山东大学科技期刊社（济南市历城区山大南路27号山东大学中心校区明德楼B座721室） 电话: 0531-88366918 E-mail: xbyxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发