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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (4): 61-64.doi: 10.6040/j.issn.1671-7554.0.2014.641

• 基础医学 • 上一篇    下一篇

β-synuclein对Ⅱ型囊泡单胺转移体表达的促进作用

杨璐1, 周英泽2, 倪明1, 樊秀双1, 满建梅1, 郭军堂1   

  1. 1. 潍坊医学院基础医学院病理生理学教研室, 山东 潍坊 261053;
    2. 四川省安岳县人民医院病理科, 四川 安岳 642350
  • 收稿日期:2014-09-24 修回日期:2014-12-24 出版日期:2015-04-10 发布日期:2015-04-10
  • 通讯作者: 郭军堂.E-mail:guojuntang@163.com E-mail:guojuntang@163.com
  • 基金资助:
    山东省自然科学基金(ZR2011HM014)

Beta-synuclein promotes the expression of vesicular monoamine transporter-2

YANG Lu1, ZHOU Yingze2, NI Ming1, FAN Xiushuang1, MAN Jianmei1, GUO Juntang1   

  1. 1. Department of Pathophysiology, School of Basic Medicine, Weifang Medical University, Weifang 261053, Shandong, China;
    2. Department of Pathology, Anyue County People's Hospital, Anyue 642350, Sichuan, China
  • Received:2014-09-24 Revised:2014-12-24 Online:2015-04-10 Published:2015-04-10

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摘要： 目的 探讨β-突触核蛋白(β-synuclein)对Ⅱ型囊泡单胺转移体(VMAT2)表达的影响.方法 通过2',7'-二氯荧光黄双乙酸盐(DCFH-DA)染色检测β-synuclein稳定表达对细胞内ROS水平的影响;采用免疫荧光双标法、免疫组织化学法及Western blotting检测VMAT2在稳定转染β-synuclein 的SH-SY5Y细胞及正常SH-SY5Y细胞中的表达情况.结果 DCFH-DA荧光染色显示,稳定表达β-synuclein的细胞的荧光强度较正常SH-SY5Y细胞明显减弱.VMAT2在稳定转染β-synuclein 的SH-SY5Y细胞中的表达水平较正常SH-SY5Y细胞明显升高. 结论 β-synuclein能够促进VMAT2的表达,减少胞浆内DA含量,抑制ROS产生,在帕金森病多巴胺神经元变性过程中对其起到保护作用.

关键词: &beta, 活性氧类物质, 电休克, -突触核蛋白, 帕金森病, Ⅱ型囊泡单胺转移体, 木僵, 精神病

Abstract: Objective To investigate the effect of β-synuclein on the expression of vesicular monoamine transporter-2(VMAT2). Methods The effects of β-synuclein stable expression on the intracellular ROS level were detected by dichlorofluorescein diacetata(DCFH-DA). The expression of VMAT2 was detected by double immunofluorescence, immunohistochemistry and Western blotting in SH-SY5Y cells which were transfected stably with β-synuclein and normal SH-SY5Y cells. Results The intracellular ROS level in the cells stably expressing β-synuclein was significantly lower than that of normal SH-SY5Y cells.The expression of β-synuclein in the SH-SY5Y cells which were stably transfected with β-synuclein was higher than that of normal SH-SY5Y cells. Conclusion β-synuclein can reduce the content of DA in cytosolic by promoting the expression of VMAT2, which can inhibit the generation of ROS. It plays an important protective role in the process of dopamine neurons' degeneration in Parkinson's disease.

Key words: Beta-synuclein, Reactive oxygen species, Parkinson's, disease, Vesicular monoamine transporter-2

中图分类号: 

  • R741
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