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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (12): 50-53.doi: 10.6040/j.issn.1671-7554.0.2014.021

• 基础医学 • 上一篇    下一篇

ALA-PDT对皮肤鳞状细胞癌A431细胞Caspase-3及Caspase-7表达的影响

刘园园1, 王逸飞2, 张春敏1, 刘瑶1, 魏国1, 张春红1, 刘瑛1   

  1. 1. 山东大学第二医院皮肤科, 山东 济南 250033;
    2. 山东大学医学院, 山东 济南 250012
  • 收稿日期:2014-01-10 修回日期:2014-11-11 发布日期:2014-12-10
  • 通讯作者: 张春敏。E-mail:cmzhang1878@163.com E-mail:cmzhang1878@163.com
  • 基金资助:
    山东省科学技术发展计划(2009GG10002026)

Effects of ALA-PDT on Caspase-3 and Caspase-7 expressions in cutaneous squamous cell carcinoma A431 cells

LIU Yuanyuan1, WANG Yifei2, ZHANG Chunmin1, LIU Yao1, WEI Guo1, ZHANG Chunhong1, LIU Ying1   

  1. 1. Department of Dermatology, The Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. School of Medicine, Shandong University, Jianan 250012, Shandong, China
  • Received:2014-01-10 Revised:2014-11-11 Published:2014-12-10

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摘要： 目的 观察盐酸氨酮戊酸散(ALA)-光动力疗法(PDT)对皮肤鳞状细胞癌A431细胞中Caspase-3及Caspase-7表达的影响。方法 体外培养A431细胞,应用MTT法检测不同ALA浓度、不同培养时间、不同PDT光照剂量以及单独加药和单独光照对A431细胞增殖与凋亡的影响,并设Hacat作为对照;应用实时荧光定量PCR及Western blotting检测A431细胞及Hacat细胞中Caspase-3及Caspase-7 mRNA和蛋白的表达水平。结果 A431细胞中Caspase-3及Caspase-7 mRNA和蛋白的表达量低于Hacat细胞,其表达强弱差异有统计学意义(P<0.05)。ALA-PDT对A431细胞的增殖具有一定的抑制作用,并引起Caspase-3及Caspase-7 mRNA和蛋白的表达量升高(P<0.01)。当光照剂量为80 J/cm2时,Caspase-3、Caspase-7 mRNA及蛋白的表达量均达到峰值。结论 ALA-PDT对皮肤鳞状细胞癌A431细胞的增殖抑制可能是通过诱导Caspase-3及Caspase-7的表达,引起细胞的凋亡从而发挥作用。

关键词: Caspase-7, 光动力疗法, Caspase-3, 皮肤鳞状细胞癌A431细胞

Abstract: Objective To investigate the effect of aminolevulinic acid hydrochloride (ALA)-photodynamic therapy (PDT) on Caspase-3 and Caspase-7 expressions in cutaneous squamous cell carcinoma A431 cells. Methods A431 cells were cultured in vitro. The proliferation and apoptosis of A431 cells with different drug concentration, different incubation time, different light dose, as well as with drug and light alone, were assessed by MTT. Hacat was as control. The mRNA expressions of Caspase-3 and Caspase-7 were detected by Real-time PCR. The protein expressions of Caspase-3 and Caspase-7 in A431 cells and Hacat cells were detected by Western blotting. Results The mRNA and protein expressions of Caspase-3 and Caspase-7 were lower in A431 cells than in the Hacat cells with statistically significant difference (P<0.05). ALA-PDT inhibited the proliferation of A431 cells in a certain extent, and increased the mRNA and protein expressions of Caspase-3 and Caspase-7 (P<0.01). The inhibition on A431 cells could reach the maximum when the light dose was 80 J/cm2 with the same incubation time and concentration of ALA. Conclusion ALA-PDT can induce the expressions of Caspase-3 and Caspase-7 directly or indirectly, which induce the apoptosis of cutaneous squamous cell carcinoma A431 cells.

Key words: Squamous cell carcinoma A431 cells, Caspase-3, Caspase-7, Photodynamic therapy

中图分类号: 

  • R75
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