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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (10): 37-41.doi: 10.6040/j.issn.1671-7554.0.2015.176

• 临床医学 • 上一篇    下一篇

二肽基肽酶6在难治性颞叶癫痫患者中的表达

赵振英1, 周雪颖2, 刘黎青2, 刘峰3, 周盛年1   

  1. 1. 山东大学齐鲁医院神经内科 山东大学脑科学研究所, 山东 济南 250012;
    2. 山东中医药大学, 山东 济南 250355;
    3. 山东省疾病预防控制中心, 山东 济南 250014
  • 收稿日期:2014-12-08 出版日期:2015-10-10 发布日期:2015-10-10
  • 通讯作者: 周盛年。E-mail:shengnianzhou163@163.com E-mail:shengnianzhou163@163.com
  • 基金资助:
    国家自然科学基金(81371439)

Altered expression of dipeptidyl peptidase 6 in patients with intractable temporal lobe epilepsy

ZHAO Zhenying1, ZHOU Xueying2, LIU Liqing2, LIU Feng3, ZHOU Shengnian1   

  1. 1. Department of Neurology, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, Jinan 250012, Shandong, China;
    2. Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China;
    3. Shandong Center for Disease Control and Prevention, Jinan 250014, Shandong, China
  • Received:2014-12-08 Online:2015-10-10 Published:2015-10-10

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摘要： 目的 探讨难治性癫痫患者颞叶皮质中二肽基肽酶6(DPP6)的表达情况。方法 在重庆医科大学建立的脑组织库中,按照随机化原则抽取27例癫痫患者的脑组织(癫痫组)和15例脑外伤患者的脑组织(对照组),应用免疫组织化学染色、蛋白质印迹杂交、RT-PCR法检测DPP6的表达水平。结果 DPP6表达于两组大脑神经元的细胞膜,蛋白质印迹杂交结果显示,癫痫组和对照组DPP6的平均光密度(OD)值分别为0.86±0.04和0.37±0.02,差异有统计学意义(P<0.001)。癫痫组和对照组脑组织DPP6 mRNA的相对表达量分别为0.74±0.03和0.35±0.03,差异有统计学意义(P<0.001)。结论 异常表达的DPP6可能参与了难治性癫痫的形成。

关键词: 突触重塑, 二肽基肽酶6, 树突棘, 难治性癫痫

Abstract: Objective To investigate the expression of dipeptidyl peptidase 6 (DPP6) in the neocortex of patients with intractable temporal lobe epilepsy (TLE) and discuss its role in the formation of intractable epilepsy. Methods DPP6 expressions were detected in 27 temporal neocortex tissue samples from patients with intractable TLE (epilepsy group)and 10 histologically normal temporal lobe tissue samples from patients without epilepsy (control group) by immunohistochemical staining, Western blotting and RT-PCR methods. Results DPP6 was found to be expressed mainly in the membrane of neurons. Western blotting analysis showed that the mean optical density (OD) ratios of DPP6 in the temporal neocortices of epilepsy group and control group were 0.86±0.04 and 0.37±0.02, respectively(P<0.001). The level of DPP6 mRNA in epilepsy group (0.74±0.03) was significantly higher than control group(0.35±0.03)(P<0.001). Conclusion The abnormal expression of DPP6 may contribute to epileptogenesis and represent a novel therapeutic target.

Key words: Synaptic plasticity, Dipeptidyl peptidase 6, Intractable epilepsy, Dendritic spine

中图分类号: 

  • R741
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