您的位置：山东大学 -> 科技期刊社 -> 《山东大学学报（医学版）》

山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (7): 13-18.doi: 10.6040/j.issn.1671-7554.0.2014.719

• 基础医学 • 上一篇    下一篇

益气活血养阴解毒方对放射性肺损伤中TGF-β1、TNF-α表达的影响

王静1, 高桂新2, 万云焱1, 刘庆华1, 姚周虹1, 林殿杰1   

  1. 1. 山东大学附属省立医院呼吸内科, 山东 济南 250021;
    2. 山东大学附属省立医院肿瘤科, 山东 济南 250021
  • 收稿日期:2014-10-17 修回日期:2015-03-27 出版日期:2015-07-10 发布日期:2015-07-10
  • 通讯作者: 林殿杰。E-mail:dianjielin@126.com;高桂新。E-mail:slggxin@163.com E-mail:dianjielin@126.com;slggxin@163.com
  • 基金资助:
    山东省自然科学基金(ZR2009CM063,2010ZRB141621B)

Effect of Yiqihuoxue-Yangyinjiedu recipe on expressions of TGF-β1 and TNF-α in radiation-induced lung injury

WANG Jing1, GAO Guixin2, WAN Yunyan1, LIU Qinghua1, YAO Zhouhong1, LIN Dianjie1   

  1. 1. Department of Respiratory Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Oncology Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2014-10-17 Revised:2015-03-27 Online:2015-07-10 Published:2015-07-10

PDF (PC)

319

摘要： 目的 研究益气活血养阴解毒方对放射性肺损伤中转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)表达的影响, 探讨其对放射性肺损伤的防治作用及机制。方法 将小鼠随机分为对照组(n=21)、中药组(n=21)、照射组(n=21)、治疗组(照射+中药, n=21), 用直线加速器对后两组小鼠全肺进行单次照射(12 Gy), 分别于照射后1、24、72 h和2、4、8、24周处死小鼠, 取标本进行组织学观察、RT-qPCR及免疫组化检测, 观察小鼠组织学变化及不同时间点的TGF-β1、TNF-α的表达。结果 益气活血养阴解毒方可以改善放射性肺损伤。照射组TGF-β1、TNF-α的mRNA及蛋白水平表达均高于对照组及中药组(P<0.05);除照射后1、24 h外, 治疗组TGF-β1、TNF-α在其余各时间点的表达水平均低于照射组(P<0.05)。结论 益气活血养阴解毒方具有抑制照射刺激下TGF-β1、TNF-α的表达、减轻放射性肺损伤的作用, 其机制与抑制TGF-β1、TNF-α的表达有关。

关键词: 放射性肺损伤, 转化生长因子-β1, 肿瘤坏死因子-α, 益气活血养阴解毒方

Abstract: Objective To investigate the influence of traditional Chinese medicine, Yiqihuoxue-Yangyinjiedu recipe, on transforming growth factor-β1(TGF-β1) and tumor necrosis factor-α(TNF-α) in lung tissues of mice with radiation-induced lung injury, and to explore the effects and action mechanism of Yiqihuoxue-Yangyinjiedu recipe on preventing and treating radiation-induced lung injury. Methods Mice were randomly divided into control group (n=21), drug group (n=21), radiation group (n=21), and treatment group (radiation+drug, n=21). The latter two groups were irradiated at whole thorax with a dose of 12 Gy. The pathology of radiation-induced lung injury was monitored. The expressions of TGF-β1 and TNF-α were analyzed by RT-qPCR and immunohistochemistry at 1, 24, 72 h and 2, 4, 8, 24 week post-irradiation. Results The results of histology demonstrated that Yiqihuoxue-Yangyinjiedu recipe treatment ameliorated radiation-induced lung injury. Expressions of TGF-β1, TNF-α mRNA and protein in radiation group were higher than those in control group and drug group (P<0.05). Except at 1 h and 24 h, expressions of TGF-β1 and TNF-α in treatment group at each time point were lower than those in radiation group (P<0.05). Conclusion Yiqihuoxue-Yangyinjiedu recipe can inhibit the expressions of TGF-β1 and TNF-α, and may play an important role in preventing and treating radiation-induced lung injury, which may be related to inhibit the expressions of TGF-β1 and TNF-α.

Key words: Radiation-induced lung injury, Tumor necrosis factor-α, Transforming growth factor-β1, Yiqihuoxue-Yangyinjiedu recipe

中图分类号: 

  • R563.1
[1] Mazeron R, Etienne-Mastroianni B, Pérol D, et al. Predictive factors of late radiation fibrosis: a prospective study in non-small cell lung cancer[J]. Int J Radiat Oncol Biol Phys, 2010, 77(1): 38-43.
[2] Oh YT, Noh OK, Jang H, et al. The features of radiation induced lung fibrosis related with dosimetric parameters[J]. Radiother Oncol, 2012, 102(3):343-346.
[3] Arkovitz MS, Garcia VF, Szabó C, et al. Decreased pulmonary compliance is an early indicator of pulmonary oxygen injury[J]. J Surg Res, 1997, 67(2): 193-198.
[4] Ashcroft T, Simpson JM, Timbrell V. Simple method of estimating severity of pulmonary fibrosis on a numerical scale[J]. J Clin Pathol, 1988, 41(4): 467-470.
[5] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4): 402-408.
[6] Friedrichs K, Gluba S, Eidtmann H, et al. Overexpression of p53 and prognosis in breast cancer[J]. Cancer, 1993, 72(12): 3641-3647.
[7] Hill RP, Zaidi A, Mahmood J, et al. Investigations into the role of inflammation in normal tissue response to irradiation[J]. Radiother Oncol, 2011, 101(1): 73-79.
[8] Kim JY, Kim YS, Kim YK, et al. The TGF-beta1 dynamics during radiation therapy and its correlation to symptomatic radiation pneumonitis in lung cancer patients[J]. Radiat Oncol, 2009, 4: 59.
[9] Sanderson N, Factor V, Nagy P, et al. Hepatic expression of mature transforming growth factor beta1 in transgenic mice results in multiple tissue lesions[J]. Proc Natl Acad Sci U S A, 1995, 92(7): 2572-2576.
[10] 黄莺, 叶燕青, 孙洁民.肺纤维化形成过程中大鼠肺成纤维细胞分泌TGF-βmRNA及HGF mRNA对肺泡II型上皮细胞的影响[J]. 重庆医学, 2010, 39(19): 2548-2549, 2552. HUANG Ying, YE Yanqing, SUN Jiemin. Effect of pulmonary fibroblast express TGF-β mRNA and HGF mRNA to II alveolar epithelial cell in the rat model of pingyangmycin induced pulmonary fibrosis[J]. Chongqing Medicine, 2010, 39(19): 2548-2549, 2552.
[11] Chung EJ, Hudak K, Horton JA, et al. Transforming growth factor alpha is a critical mediator of radiation lung injury[J]. Radiat Res. 2014, 182(3): 350-362.
[12] 高桂新, 王成祥.周平安教授治疗放射性肺炎的临床经验[J].北京中医药大学学报:中医临床版, 2009, 16(4): 24-25.
[13] Dong XR, Wang JN, Liu L, et al. Modulation of radiation-induced tumour necrosis factor-α and transforming growth factor β1 expression in the lung tissue by Shengqi Fuzheng injection[J]. Mol Med Rep, 2010, 3(4): 621-627.
[14] Zhang K, Yang S, Zhu Y, et al. Protection against acute radiation-induced lung injury: a novel role for the anti-angiogenic agent Endostar[J]. Mol Med Rep, 2012, 6(2): 309-315.
[15] Wang J, Xu HW, Li BS, et al. Preliminary study of protective effects of flavonoids against radiation-induced lung injury in mice[J]. Asian Pac J Cancer Prev, 2012, 13(12): 6441-6446.
[16] Xie CH, Zhang MS, Zhou YF, et al. Chinese medicine Angelica sinensis suppresses radiation-induced expression of TNF-alpha and TGF-beta1 in mice[J]. Oncol Rep, 2006, 15(6): 1429-1436.
[17] 谢丛华, 周云峰, 彭纲, 等.当归对小鼠放射性肺损伤过程中TNF-α水平的影响[J].中华放射肿瘤学杂志, 2005, 14(1): 59-63. XIE Conghua, ZHOU Yunfeng, PENG Gang, et al. Modulation by Angelia Sinensis the expression of tumor necrosis factor αin radiation-induced damage of the lung[J]. Chin J Radiat Oncol, 2005, 14(1): 59-63.
[1] 唐蒙蒙, 金女娃, 刘传振, 刘凯, 曹广庆, 王鹤, 庞昕焱, 吴树明. 丙酮酸乙酯对高动力性肺高压的治疗作用[J]. 山东大学学报（医学版）, 2015, 53(5): 75-80.
[2] 蔺晨, 郭芳, 季维娜, 于文成. 社区获得性肺炎患者的外周血TNF-α、sTREM-1、α1-APG水平[J]. 山东大学学报（医学版）, 2015, 53(2): 61-64.
[3] 张颖哲, 吴东进, 彭长亮, 李波翰, 宋扬, 张程, 赵杰, 李德全, 杨中雁, 刘鹏, 赵坤, 马胜忠, 高春正. siRNA抑制转化生长因子-β1对小鼠黄韧带骨化的影响[J]. 山东大学学报（医学版）, 2014, 52(8): 27-33.
[4] 张锦航1，张鲁伟1，刘新农2，田军1. Db/db小鼠肾脏中Tribble3的表达及其与肾脏纤维化的关系[J]. 山东大学学报（医学版）, 2014, 52(2): 38-43.
[5] 孙越,刘香岚,王群,郭春,石永玉,张利宁. 人TIPE家族新成员TIPE3特异性抗体的鉴定及应用[J]. 山东大学学报（医学版）, 2014, 52(1): 5-9.
[6] 韩晓娟1，马学强2，杜怡峰1. 海风藤提取物对Aβ寡聚体激活的小胶质细胞释放炎性因子的抑制作用[J]. 山东大学学报(医学版), 2013, 51(5): 6-10.
[7] 刘丹丹1,高聆2，赵家军1，韩文霞1. 健脾益肾、活血化瘀中药复方对高糖刺激下肾小球系膜细胞增殖的影响[J]. 山东大学学报(医学版), 2013, 51(5): 29-32.
[8] 刘娜，蔡可丽. Ang (1-7)及培哚普利对糖尿病视网膜TGF-β1表达的影响[J]. 山东大学学报(医学版), 2013, 51(5): 62-65.
[9] 迟蔚蔚1，高海燕2，高海东3. 转化生长因子β1诱导的跨膜蛋白参与乳腺癌发展的分子机制[J]. 山东大学学报(医学版), 2013, 51(4): 26-29.
[10] 徐慧1，王义国2，刘长虹2，刘倩2，杜娟3. HBV在HK-2细胞内的表达及对其转分化的影响[J]. 山东大学学报(医学版), 2013, 51(3): 15-20.
[11] 王玮1,李鲁杨2,孟晓慧1. 甘草酸二胺联合前列地尔对大鼠
肾间质纤维化的影响[J]. 山东大学学报(医学版), 2012, 50(9): 29-.
[12] 韩艳鑫，丛雅琴，王志敏. 骨髓增生异常综合征患者骨髓间充质干细胞的免疫抑制作用[J]. 山东大学学报(医学版), 2012, 50(6): 97-.
[13] 王月恒1，邢毅2，王玖玲1，代军3，李淑玲1. 多囊卵巢综合征患者血清内脂素及TNF-α的水平变化及其意义[J]. 山东大学学报(医学版), 2012, 50(2): 94-.
[14] 姚丽1，孙钦峰2，杨春惠1. 子痫前期伴牙周炎患者血清TNF-α、IL-6及CRP水平的检测[J]. 山东大学学报(医学版), 2012, 50(10): 123-126.
[15] 焦齐1,2,王伟3,范全心4. 不同滤出量平衡超滤对体外循环手术中炎性介质水平的影响[J]. 山东大学学报(医学版), 2011, 49(8): 136-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2018 《山东大学学报(医学版)》编辑部　
地址:山东大学科技期刊社（济南市历城区山大南路27号山东大学中心校区明德楼B座721室） 电话: 0531-88366918 E-mail: xbyxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发