关键词: 神经元, 诱导分化, 神经前体细胞, 胚胎干细胞, 孤雌生殖

Abstract: To investigate the feasibility of direct differentiation of mouse parthenogenetic stem cells into neural cells without embryonic body culture in vitro. MethodsThe method of phase induction was used to induce direct differentiation from parthenogenetic stem cells to neural precursors which were identified by nestin immunofluorescence staining. Sequential culture was applied for the further differentiation into neural cells with the removal of mitogen and addition of 5% fetal bovine serum. The characteristics of the further induced cells were identified by immunofluorescence staining. ResultsLarge amounts of parthenogenetic stem cells were differentiated into neural precursors, which were nestin-positive in the selected medium on the 3rd day. Also β-Ⅲ-tubline-positive neural cells could be induced from the neural precursors by further culture. ConclusionMouse parthenogenetic stem cells could be directly differentiated into neural cells without embryonic body culture in vitro and induce large amounts of neural precursors.

Key words: Parthenogenesis, Embryonic stem cells, Inducing differentiation, Neural precursor, Neurons