绞股蓝总皂苷对谷氨酸所致大鼠海马组织损伤的保护作用

山东大学学报(医学版)

绞股蓝总皂苷对谷氨酸所致大鼠海马组织损伤的保护作用

韩玉霞¹,魏欣冰¹,李霞²,丁燕¹,辛华²,丁华¹

山东大学医学院 1. 药理学研究所； 2.细胞生物学研究所，济南 250012

收稿日期:2007-12-28 修回日期:1900-01-01 出版日期:2008-05-16 发布日期:2008-05-16
通讯作者: 韩玉霞

Protective effects of gypenosides against glutamate-induced hippocampus injury in rats

HAN Yu-xia¹, WEI Xin-bing¹,　LI Xia², DING Yan¹, XIN Hua², DING Hua¹

1. Institute of Pharmacology，School of Medicine, Shangdong University, Jinan 250012, China;2. Institute of Cell Biology，School of Medicine， Shangdong University, Jinan 250012, China

Received:2007-12-28 Revised:1900-01-01 Online:2008-05-16 Published:2008-05-16
Contact: HAN Yu-xia

摘要/Abstract

摘要： 目的探讨绞股蓝总皂苷(gypenosides, GP)对大鼠侧脑室注射谷氨酸(glutamate, Glu)引起的海马组织氧化损伤的保护作用。方法将大鼠随机分为假手术组、Glu损伤组、GP低（200mg/kg）、中（400mg/kg）、高（800mg/kg）剂量组。GP组预先灌胃给药10d。大鼠经侧脑室注射Glu后2h取脑，测定各组大鼠海马组织匀浆中的丙二醛(MDA)含量、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶(GSH-Px) 活性及产生羟自由基的能力;制备海马组织切片，观察各组形态学变化。结果Glu损伤组大鼠海马组织中MDA含量和羟自由基生成能力均高于假手术组(P＜0.05)，而SOD和GSH-Px活性降低(P＜0.05)；与Glu损伤组比较，GP组MDA含量和产生羟自由基的能力降低(P＜0.01)，SOD和GSH-PX活性明显增强(P＜0.01)；HE染色显示, GP组海马神经元变性及坏死较Glu损伤组明显减少。GP 400mg/kg对Glu所致海马组织氧化损伤的保护作用最为明显。结论GP通过增强抗氧化酶活性，抑制羟自由基和脂质过氧化物生成而减轻Glu介导的氧化性神经损伤，发挥神经保护作用。

关键词: 氧化性神经毒性, 谷氨酸, 绞股蓝总皂苷

Abstract: To investigate the protective effects of gypenosides(GP) against glutamate-induced oxidative injury to the hippocampus of rats. Methods60 Wistar rats were randomly divided into 5 groups: the sham-operation group, the glutamate-induced injury group, the GP low (200mg/kg), medium(400mg/kg) and high (800mg/kg) dose groups. Intra-gastric administration of GP was used for 10d. 2h after injection with glutamate through the lateral ventricle, the homogenate of the hippocampus was collected and the MDA, SOD, GSH-Px activity and the hydroxyl radical generation capacity were determined. Results: The MDA concentration and hydroxyl radical generation capacity were significantly increased(P＜0.05), however activities of SOD and GSH-Px were significantly decreased (P＜0.01)in the glutamate-induced injury group compared with the sham-operation group. Also, the MDA concentration and hydroxyl radical generation capacity were significantly decreased in the GP groups compared with the glutamate-induced injury group (P＜0.01), and activities of SOD and GSH-Px were significantly increased (P＜0.01) by GP treatments, and 400mg/kg GP had the most effective action. ConclusionThe protective effects of GP against Glu-mediated nerve injury may be mediated by increasing the activity of antioxidant enzymes and inhibiting the hydroxyl radical production.

Key words: Gypenosides, Glutamate, Oxidative neurotoxicity

中图分类号:

R965

韩玉霞,魏欣冰,李霞,丁燕,辛华,丁华 . 绞股蓝总皂苷对谷氨酸所致大鼠海马组织损伤的保护作用[J]. 山东大学学报(医学版), 2008, 46(5): 449-452.

HAN Yu-xia,WEI Xin-bing,LI Xia,DING Yan,XIN Hua,DING Hua. Protective effects of gypenosides against glutamate-induced hippocampus injury in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(5): 449-452.

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