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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (12): 5-9.doi: 10.6040/j.issn.1671-7554.0.2014.190

• 基础医学 • 上一篇    下一篇

封闭内源性NT-3在脊髓损伤后期神经元有髓神经纤维出芽中的作用

王旭阳1,2, 江敦清1, 陈世文1, 田恒力1, 刘芬2, 李志强3   

  1. 1. 上海交通大学附属第六人民医院神经外科, 上海 200233;
    2. 温州医科大学基础医学院, 浙江 温州 325000;
    3. 上海交通大学附属第六人民医院南院神经外科, 上海 201499
  • 收稿日期:2014-04-03 修回日期:2014-11-24 发布日期:2014-12-10
  • 通讯作者: 李志强。E-mail:lzq_999@163.com;田恒力。E-mail:ltianhengli@163.com E-mail:lzq_999@163.com;ltianhengli@163.com
  • 基金资助:
    上海市卫生局课题(2011351);浙江省自然科学基金(Y2090864)

Function of endogenous NT-3 in the regeneration sprouting of large neuron at late-stage spinal cord injury

WANG Xuyang1,2, JIANG Dunqing1, CHEN Shiwen1, TIAN Hengli1, LIU Fen2, LI Zhiqiang3   

  1. 1. Department of Neurosurgery, Shanghai Jiao Tong University Affiliated 6th People's Hospital, Shanghai 200233, China;
    2. School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China;
    3. Department of Neurosurgery, South Campus of Shanghai Jiao Tong University Affiliated 6th People's Hospital, Shanghai 201499, China
  • Received:2014-04-03 Revised:2014-11-24 Published:2014-12-10

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摘要： 目的 探讨成年猫脊髓损伤后期内源性Neurotrophin-3 (NT-3) 对脊髓背根节(DRG)大型感觉神经元功能修复的作用。方法 切除猫L1-L5、L7-S2 DRG,术后随机行NT-3抗体封闭处理,2个月后行形态学示踪和免疫组化、酶组化等比较分析。结果 NT-3阳性神经元在DRG和脊髓的分布和损伤后变化与既往研究一致,霍乱毒素B亚单位结合辣根过氧化物酶复合物(CB-HRP)示踪显示,手术组DRG感觉性大神经元中枢投射轴突终末集中分布在Ⅲ、Ⅳ、Ⅴ 板层内侧,其有髓神经纤维终末再生出芽点密度高于对照组,NT-3抗体封闭组轴突再生较手术组显著降低。结论 脊髓损伤后期修复中,内源性NT-3能促进损伤脊髓和感觉神经元的形态和功能修复,有利于脊髓后角Ⅲ、Ⅳ、Ⅴ板层有髓神经纤维轴突终末的再生、出芽生长,其机制可能在于上调DRG大型神经元NT-3的表达,进而参与脊髓可塑性修复。

关键词: 大型感觉神经元, 神经营养因子-3, 形态学示踪, 神经再生, 抗体封闭

Abstract: Objective To explore the function of endogenous neurotrophin-3 (NT-3) in the regeneration sprouting of large neuron at late-stage spinal cord following dorsal root rhizotomy in adult cats. Methods The 1st to the 5th lumbar (L1-L5), and the 7th lumbar (L7) to the 2nd sacral (S2) dorsal root ganlia (DRG) were exposed and removed from their respective intervertebral foramina, leaving the L6 DRG untouched. Antibody of NT-3 was blocked. Two months later, CB-HRP transganglionic tracing, histochemistry and enzymohistochemistry analysis were performed to compare the spinal cord segments and the spared DRG. Results NT-3 positive immunity reaction (IR) mainly distributed in large-sized neurons of DRG and part of neurons in spinal lamina II. Two months after the operation, the number of NT-3-IR neurons in DRG significantly increased (P<0.05), but in lamina Ⅱ there was no difference compared to the control group. After CB-HRP tetramethyl benzidine (TMB) enzymohistochemistry coloration, the densities of axon terminalsprouting derived from spared DRG were more markedly increased in the operation group than in the control group. However, after antibody blockade of NT-3, the densities decreased sharply compared to that of operation group. Conclusion The regeneration sprouting of large sensory neuron of DRG at late-stage distribute mainly in the deep layers (Ⅲ, Ⅳ, Ⅴ layer) of spinal dorsal horn. Endogenous NT-3 may be involved in the spinal cord plasticity by up-regulating the expression of NT-3, which promotes the long-term repair of injured spinal cord.

Key words: Morphological tracing, Large sensory neuron, Antibody blockade, Neural Regeneration, Neurotrophin-3

中图分类号: 

  • R323.85
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