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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (9): 44-47.doi: 10.6040/j.issn.1671-7554.0.2014.217

• 基础医学 • 上一篇    下一篇

大黄素对角膜炎大鼠角膜组织NF-κB活化表达的影响及意义

陈国玲1, 任然2, 张英辉3, 宋玮1, 考欣1, 张晗1   

  1. 1. 山东大学第二医院眼科, 山东 济南 250033;
    2. 济南市第五人民医院放射科, 山东 济南 250022;
    3. 山东大学第二医院病案室, 山东 济南 250033
  • 收稿日期:2014-04-12 修回日期:2014-06-06 出版日期:2014-09-10 发布日期:2014-09-10
  • 基金资助:
    山东省教育厅科技计划项目(J08LH59)

Significance and effect of emodin on activation of nuclear factor-κB in corneas of keratitis rats

CHEN Guoling1, REN Ran2, ZHANG Yinghui3, SONG Wei1, KAO Xin1, ZHANG Han1   

  1. 1. Department of Ophthalmology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Department of Radiology, the 5th People's Hospital of Jinan, Jinan 250022, Shandong, China;
    3. Department of Medical Records, the Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2014-04-12 Revised:2014-06-06 Online:2014-09-10 Published:2014-09-10
  • Contact: 张晗。E-mail:zhanghan9157@gmail.com E-mail:zhanghan9157@gmail.com

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摘要： 目的 观察大黄素对角膜炎大鼠角膜组织核因子-κB（NF-κB）活化表达的影响及意义。方法 制备角膜炎动物模型。模型制备前30 min，分别于治疗组和炎症组大鼠结膜下注射大黄素及溶剂。裂隙灯显微镜观察大鼠眼部变化；Western blotting检测评价角膜组织NF-κB活化表达；免疫组织化学法检测角膜组织细胞间黏附分子-1 （ICAM-1）蛋白的表达。结果 成功制备了角膜炎大鼠模型，大黄素可改善角膜炎大鼠的眼部炎症反应，减少角膜组织炎细胞浸润。治疗组κB抑制蛋白α（IκBα）在脂多糖（LPS）刺激后各时点的表达均高于同时点炎症组表达（P<0.01）。LPS刺激可促进ICAM-1在角膜组织的阳性表达，该作用可被大黄素部分抑制。结论 大黄素可部分抑制角膜炎大鼠角膜组织中NF-κB活化，有效减轻角膜炎症的损伤。

关键词: 核转录因子-κB, 细胞因子, 角膜炎, 大黄素

Abstract: Objective To investigate the significance and effect of emodin on the activation of nuclear factor-κB (NF-κB) in corneas of keratitis rats. Methods Pseudomonas aeruginosa lipopolysaccharide (LPS)-induced keratitis models were established in Wistar rats. Before LPS exposure (30 minutes), emodin and its vehicle were injected into subconjunctival tissues of rats in treatment group and inflammation group. At different time after LPS challenge, rats were examined with slit lamp microscope to observe the severity of ocular inflammation. The expression of inhibitor NF-κBα (IκBα) was determined with Western blotting, and intercellular adhesion molecule-1 (ICAM-1) was detected with immunohistochemical staining. Results The rat models of keratitis were successfully established. LPS could induce a severe corneal inflammatory response, which could be improved by emodin. IκBα level was significantly decreased after LPS exposure, and the degeneration of IκBα induced by LPS was markedly inhibited by pretreatment with emodin (P<0.01). Furthermore, the positive cells of ICAM-1 staining were significantly decreased in treatment group as compared with inflammation group. Conclusion LPS takes part in the activation of NF-κB in keratitis corneas, and emodin can partly inhibit this activation and result in therapeutic effect.

Key words: Emodin, Cytokines, Nuclear factor-κB, Keratitis

中图分类号: 

  • R772.2
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