贵州地区临床酵母菌种类组成和分布

doi:10.6040/j.issn.1671-7554.0.2014.141

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (8): 94-97.doi: 10.6040/j.issn.1671-7554.0.2014.141

贵州地区临床酵母菌种类组成和分布

王梅竹¹, 曹煜², 赵亮¹, 徐小珊³, 张佩芳³, 吕倩¹, 白逢彦⁴, 康颖倩¹

1. 贵阳医学院微生物学教研室, 贵州贵阳 550004;
2. 贵阳医学院附属医院皮肤性病科, 贵州贵阳 550004;
3. 贵阳市白云区人民医院妇产科, 贵州贵阳 550043;
4. 中国科学院微生物研究所真菌学国家重点实验室, 北京 100101

收稿日期:2014-03-14 修回日期:2014-06-18 出版日期:2014-08-10 发布日期:2014-08-10
通讯作者: 康颖倩。E-mail：joycekangtokyo@gmail.com E-mail:joycekangtokyo@gmail.com
基金资助:
国家自然科学基金地区科学基金项目（31060006，31260029），贵州省科技厅社会发展科技攻关项目（黔科合SY字[2011]3017号）；贵州省卫生厅科技项目（gzwkj2010-1-025）；贵阳市科技局社会发展与民生计划（筑科合同[2011103]16号）

Composition and distribution of clinical yeasts in Guizhou region, China

WANG Meizhu¹, CAO Yu², ZHAO Liang¹, XU Xiaoshan³, ZHANG Peifang³, LV Qian¹, BAI Fengyan⁴, KANG Yingqian¹

1. Department of Microbiology, Guiyang Medical College, Guiyang 550004, Guizhou, China;
2. Department of Dermatology and Venereal Disease, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou, China;
3. Department of Obstetrics and Gynecology, Baiyun People's Hospital, Guiyang 550014, Guizhou, China;
4. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Received:2014-03-14 Revised:2014-06-18 Online:2014-08-10 Published:2014-08-10

摘要/Abstract

摘要： 目的调查贵州地区临床常见病原真菌的种类及其分布特点。方法收集来源于贵州地区各种系统性疾病（深部组织来源）和外阴阴道念珠菌病（VVC）患者的临床酵母菌248株，根据CHROMagar培养基鉴定结果，进行基于ITS1区单链构象多态性（SSCP）图谱和序列分析的分子生物学菌种鉴定。结果分子生物学鉴定结果显示，收集的菌种主要来源于2个属10个种，其中优势属为Candida，优势种为C.albicans；分子生物学与CHROMagar培养基鉴定结果的符合率为91.53%；贵州地区深部组织与VVC来源的临床酵母菌的菌相分布具有显著差异。结论 ITS1区SSCP图谱结合序列测定分析在酵母菌种鉴定中是一种准确、可靠的方法。C.albicans仍为贵州地区的主要病原真菌，但其他酵母菌感染较以往报告呈增多趋势，特别是VVC来源的C.glabrata比例明显高于全国平均水平。

关键词: 单链构象多态性, 酵母菌, 菌种鉴定, 贵州

Abstract: Objectives To survey the species composition and distribution of clinical yeasts in Guizhou region. Methods Morphological observation, ITS1-SSCP patterning and sequencing were performed to identify 248 yeast strains from different clinical origins in Guizhou region, China. Results The identification result suggested that the isolated strains came from 2 genus and 10 species, in which the dominant genus and species were Candida and C.albicans, respectively. The molecular identification and CHROMagar testing coincidence rate was 91.53%. The distribution of different sources of clinical yeasts was significantly different in Guizhou region. Conclusion The combination of ITS1-SSCP and sequencing analyses can be accuracy and reliable when identifying the clinical yeasts with similar phenotypes. Although the most popular clinical yeast species was still C.albicans in local area, the increasing trend of non-albicans Candida species was noticed in this study, especially C.glabrata strains isolated from vulvovaginal andidiasis (VVC) patients.

Key words: Yeast, Strain identification, Guizhou, Single-strand conformation polymorphism

中图分类号:

R379

王梅竹, 曹煜, 赵亮, 徐小珊, 张佩芳, 吕倩, 白逢彦, 康颖倩. 贵州地区临床酵母菌种类组成和分布[J]. 山东大学学报（医学版）, 2014, 52(8): 94-97.

WANG Meizhu, CAO Yu, ZHAO Liang, XU Xiaoshan, ZHANG Peifang, LV Qian, BAI Fengyan, KANG Yingqian. Composition and distribution of clinical yeasts in Guizhou region, China[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 94-97.

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贵州地区临床酵母菌种类组成和分布

Composition and distribution of clinical yeasts in Guizhou region, China

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