您的位置：山东大学 -> 科技期刊社 -> 《山东大学学报（医学版）》

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (10): 103-107110.doi: 10.6040/j.issn.1671-7554.0.2014.422

• 临床医学 • 上一篇    下一篇

贵州地区深部感染来源白念珠菌的微卫星多态性

王梅竹1, 刘水清2, 马凯3, 刘娟3, 刘涛华1, 白逢彦4, 康颖倩1   

  1. 1. 贵阳医学院微生物学教研室, 贵州 贵阳 550004;
    2. 贵阳市第五人民医院感染一科, 贵州 贵阳 550004;
    3. 贵阳中医学院第二附属医院检验科, 贵州 贵阳 550043;
    4. 中国科学院微生物研究所真菌学国家重点实验室, 北京 100101
  • 收稿日期:2014-06-27 修回日期:2014-09-17 出版日期:2014-10-10 发布日期:2014-10-10
  • 通讯作者: 康颖倩。E-mail：joycekangtokyo@gmail.com E-mail:joycekangtokyo@gmail.com
  • 基金资助:
    国家自然科学基金地区科学基金（31060006，31260029）；贵州省科技厅社会发展科技攻关项目（黔科合SY字[2011]3017号）；贵州省卫生厅科技项目（gzwkj2010-1-025）；贵阳市科技局社会发展与民生计划（筑科合同[2011103]16号）

Microsatellite polymorphism of Candida albicans isolated from invasive infection sources in Guizhou region, China

WANG Meizhu1, LIU Shuiqing2, MA Kai3, LIU Juan3, LIU Taohua1, BAI Fengyan4, KANG Yingqian1   

  1. 1. Department of Microbiology, Guiyang Medical College, Guiyang 550004, Guizhou, China;
    2. Department of Infectious Disease, the Fifth People's Hospital of Guiyang, Guiyang 550004, Guizhou, China;
    3. Department of Clinical Laboratory, Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550003, Guizhou, China;
    4. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2014-06-27 Revised:2014-09-17 Online:2014-10-10 Published:2014-10-10

PDF (PC)

125

摘要： 目的 调查贵州地区各类深部感染来源的白念珠菌CAI区微卫星基因型分布特点。方法 收集95株贵州地区深部感染来源的白念珠菌，采用CAI区的单链构型多态性（SSCP）图谱结合GeneScan分析进行CAI区微卫星分型；计算多态性信息含量（PIC）和Nei氏遗传距离，以类平均法（UPGMA）进行聚类分析。结果 95株贵州地区深部感染来源的白念珠菌被鉴定为40种CAI区微卫星基因型，其中有7种属至今尚未报道过的CAI区微卫星基因型；PIC为0.904 6；根据白念珠菌CAI区微卫星基因型间的Nei氏遗传距离将菌株分为7大类群，其中慢性阻塞性肺疾病和获得性免疫缺陷综合征（AIDS）来源白念珠菌部分菌株在某一类群呈集中分布，其余部分菌株在各类群中呈散在分布，而肿瘤来源菌株则在聚类分析中呈相对集中分布的特点。结论 贵州地区深部感染不同来源的白念珠菌菌株具有丰富的遗传多样性；不同来源的白念珠菌的CAI区微卫星基因型在微生态进化上具有相关性。

关键词: CAI区微卫星基因型, 深部感染来源, 贵州, 白念珠菌

Abstract: Objective To survey the microsatellite polymorphism of Candida albicans(C.albicans) strains isolated from invasive infection sources in Guizhou region. Methods Ninety-five independent C.albicans strains isolated from invasive infection sources in Guizhou region were investigated based on single-strand conformation polymorphisms (SSCP) and GeneScan analysis. Genotypes were identified by microsatellite locus CAI polymorphism analysis. The polymorphic information content (PIC) and Nei's genetic distance were computed by software PowerMarker V3.25. In addition, phylogenetic tree was constructed based on UPGMA methods. Results Among the 95 C.albicans strains isolated from invasive infection sources, 40 distinct CAI genotypes with various patterns were identified by GeneScan analysis, and there were 7 novel CAI microsatellite genotypes which were identified in Guizhou province. The PIC was0.904 6. Seven clusters were divided in the UPGMA phylogenetic tree with Nei's the genetic distance, in which the strains from chronic obstructive pulmonary disease and AIDS were scattered in different clusters, while strains from tumor source were gathered in some relatively close clusters. Conclusion The C.albicans strains isolated from various invasive disease sources shows abundant genetic diversity characteristics. The CAI microsatellite genotypes of C.albicans from different sources are correlated to their micro-ecological evolution evidence.

Key words: Invasive infection sources, CAI microsatellite genotype, Candida albicans Guizhou

中图分类号: 

  • R379
[1] Karahan Z C, Güriz H, Ağirbaşli H, et al. Genotype distribution of Candida albicans isolates by 25S intron analysis with regard to invasiveness[J]. Mycoses, 2004, 47(11-12): 465-469.
[2] Li J, Bai F Y. Single-strand conformation polymorphism of microsatellite for rapid strain typing of Candida albicans[J]. Med Mycol, 2007, 45(7): 629-635.
[3] 王梅竹, 曹煜, 赵亮, 等. 贵州地区临床酵母菌种类组成和分布[J]. 山东大学学报:医学版, 2014, 52(8):94-97.
WANG Meizhu, CAO Yu, ZHAO Liang, et al. Composition and distribution of clinical yeasts in Guizhou region, China[J]. Journal of Shandong University: Health Sciences, 2014, 52(8):94-97.
[4] Avni T, Leibovici L, Paul M. PCR diagnosis of invasive candidiasis: systematic review and meta-analysis[J]. J Clin Microbiol, 2011, 49(2): 665-670.
[5] Wang Q M, Li J, Wang S A, et al. Rapid differentiation of phenotypically similar yeast species by single-strand conformation polymorphism analysis of ribosomal DNA[J]. Appl Environ Microbiol, 2008, 74(9): 2604-2611.
[6] Botstein D, White R L, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. Am J Hum Genet, 1980, 32(3): 314-331.
[7] Nei M. Selectionism and neutralism in molecular evolution[J]. Mol Biol Evol, 2005, 22(12): 2318-2342.
[8] Liu K, Muse S V. PowerMarker: an integrated analysis environment for genetic marker analysis[J]. Bioinformatics, 2005, 21(9): 2128-2129.
[9] Sampaio P, Gusmão L, Alves C, et al. Highly polymorphic microsatellite for identification of Candida albicans strains[J]. J Clin Microbiol, 2003, 41(2): 552-557.
[10] Loenen W A, Dryden D T, Raleigh E A, et al. Highlights of the DNA cutters: a short history of the restriction enzymes[J]. Nucleic Acids Res, 2014, 42(1): 3-19.
[11] Du Q, Gong C, Pan W, et al. Development and application of microsatellites in candidate genes related to wood properties in the Chinese white poplar (Populus tomentosa Carr.)[J]. DNA Res, 2013, 20(1): 31-44.
[1] 王梅竹, 曹煜, 赵亮, 徐小珊, 张佩芳, 吕倩, 白逢彦, 康颖倩. 贵州地区临床酵母菌种类组成和分布[J]. 山东大学学报（医学版）, 2014, 52(8): 94-97.
[2] 邢春燕,杨艳平,庞龙滨,李军,王欣,周锦慧. 定量PCR对兔肺白念珠菌病诊断价值和标准的动物实验研究[J]. 山东大学学报(医学版), 2007, 45(9): 886-890.
[3] 苏英,李春阳. 敏感和耐药白念珠菌磷脂酶活力与其毒力关系的研究[J]. 山东大学学报(医学版), 2007, 45(5): 535-537.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2018 《山东大学学报(医学版)》编辑部　
地址:山东大学科技期刊社（济南市历城区山大南路27号山东大学中心校区明德楼B座721室） 电话: 0531-88366918 E-mail: xbyxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发