大一女生自伤行为与心理-社会-基因多态性的关联分析

doi:10.6040/j.issn.1671-7554.0.2021.0470

摘要/Abstract

摘要： 目的利用5-羟色胺受体基因单核苷酸多态性(SNPs)、社会人口学特征以及心理学特征等多模态数据,探索大一女性学生自伤行为的影响因素。方法采用 iMLDR^TM多重SNP分型,检测 68 例自伤大一女性学生(自伤组)和46 例健康对照(对照组)的5-羟色胺1A受体(HTR1A)基因4个SNP位点:rs878567、rs10042486、rs1364043、rs1423691,以及5-羟色胺2A受体(HTR2A)基因9个SNP位点:rs3125、rs1745837、rs1928042、rs2770296、rs731779、rs6313、rs2070037、rs6311、rs2070040。对基因型、等位基因及单体型频率进行比较,分析基因-心理社会-自伤交互作用。结果自伤组与对照组在精神疾病家族史、SCID诊断、冲动性人格、攻击性人格以及网络成瘾行为间差异有统计学意义(P<0.05)。13个候选SNP位点基因型与等位基因频率在自伤组和对照组中分布差异无统计学意义(P>0.05)。rs1364043位点的显性模型显示,携带G/T-T/T基因型自伤风险为对照组的2.19倍(95%CI=1.01~4.74)。HTR1A基因4个SNP位点rs10042486-rs1364043-rs1423691-rs878567以及HTR2A基因6个位点rs2770296-rs731779-rs2070037-rs2070040-rs6313-rs6311分别显示出强连锁不平衡,其构成的单体型在自伤组与对照组中差异无统计学意义(P>0.05,校正年龄、经济水平、BMI、慢性躯体疾病、精神疾病家族史、SCID诊断、攻击性人格、冲动性人格、网络成瘾行为)。rs6313、rs6311位点与冲动性人格交互作用在对照组和自伤组间差异有统计学意义,rs10042486、rs1423691、rs878567位点与网络成瘾行为和SCID诊断交互作用后,在对照组和自伤组间差异有统计学意义(P<0.05)。结论 HTR1A和HTR2A基因多态性与青年女性的自伤行为有关,或通过与冲动性人格、网络成瘾行为或精神疾病诊断交互作用而间接发挥作用。

关键词: 女性, 大学生, 自伤, 心理社会因素, 单核苷酸多态, 5-羟色胺1A受体, 5-羟色胺2A受体

Abstract: Objective To explore the factors associated with self-harm behavior in female freshmen by using multi-modal data such as single nucleotide polymorphisms(SNPs)of serotonin receptor gene, social demographic characteristics, and psychological characteristics. Methods iMLDR^TM multiplex SNP typing was used to detect 4 SNPs, i. e., rs878567, rs10042486, rs1364043, and rs1423691, in HTR1A gene and 9 SNPs, i. e., rs3125, rs1745837, rs1928042, rs2770296, rs731779, rs6313, rs2070037, rs6311, and rs2070040, in HTR2A gene from 68 female freshmen with self-harm behavior(self-harm group)and 46 healthy controls(control group). The genotypes, alleles, and haplotype frequencies were compared. Additionally, the gene-psychosocial-self-harm interaction was analyzed. Results There were significant differences in the family history of mental illness, structured clinical interview for SCID diagnosis, impulsive personality, aggressive personality, and internet addiction behavior between the self-harm and control group(P<0.05). There was no significant difference in the frequencies of genotypes and alleles of the 13 candidate SNPs between the self-harm and control groups. In the dominant model, the G/T-T/T genotype in rs1364043 locus in self-harm group was 2.19(95%CI=1.01-4.74)times of that in the control group. rs10042486-rs1364043-rs1423691-rs878567 in HTR1A gene and rs2770296-rs731779-rs2070037-rs2070040-rs6313-rs6311 in HTR2A gene showed strong linkage disequilibrium, respectively. There was no significant difference in haplotypes between the self-harm and control groups(P>0.05, adjusted for age, economic level, BMI, chronic somatic disease, family history of mental illness, SCID diagnosis, aggressive personality, impulsive personality, and internet addiction). The interaction between rs6313 and rs6311 loci and impulsive personality showed a significant difference between the control and self-harm groups. Similarly, after the interaction of rs10042486, rs1423691, and rs878567 loci with internet addiction behavior and SCID diagnosis, respectively, significant differences have been shown between the self-harm and control groups(P<0.05). Conclusion The SNPs of the HTR1A and HTR2A gene are related to the self-harm behavior in the female freshmen or play an indirect role through interaction with impulsive personality, internet addiction, or diagnosis of mental disorders.

Key words: Female, University students, Self-harm, Psychosocial factors, Single nucleotide polymorphisms, HTR1A, HTR2A

中图分类号:

C320.57

徐小明,曾琪,操军,陈小容,吕臻,王我,艾明,孔裔婷,况利. 大一女生自伤行为与心理-社会-基因多态性的关联分析[J]. 山东大学学报 (医学版), 2021, 59(11): 84-92.

XU Xiaoming, ZENG Qi, CAO Jun, CHEN Xiaorong, LYU Zhen, WANG Wo, AI Ming, KONG Yiting, KUANG Li. Associations between self-harm behavior and psychosocial-polymorphism in female freshmen[J]. Journal of Shandong University (Health Sciences), 2021, 59(11): 84-92.

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