高脂膳食、硒对大鼠抗氧化功能的影响

doi:10.6040/j.issn.1671-7554.0.2020.1358

摘要/Abstract

摘要： 目的探讨在高脂膳食模式、不同安全剂量的硒暴露情况下,硒对大鼠抗氧化功能的影响。方法选取SPF级Wistar大鼠60只,6周龄,雌雄各半,采用完全随机化法分为普通组、普通+0.3 mg/kg Se组、普通+0.9 mg/kg Se组、高脂组、高脂+0.3 mg/kg Se组和高脂+0.9 mg/kg Se组,每组10只,每两周测量一次大鼠体质量,喂养实验动物8周后,处死各组大鼠,留取样本,测定血清硒浓度及谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、总谷胱甘肽(T-GSH)等抗氧化指标。采用析因设计的方差分析进行统计学分析。结果雄鼠中,GSH-Px活力主要受硒浓度的影响(P=0.002)。膳食因素、硒浓度均能够影响SOD的活力(P=0.005,P=0.050)。T-GSH含量主要受膳食因素的影响(P<0.001)。在高脂膳食雄鼠中,随着饲料硒含量递增,大鼠血清GSH-Px活力、T-GSH含量先升高后下降。结论一定程度上,低、中浓度硒促进高脂膳食雄鼠的抗氧化功能,随着硒浓度进一步升高,大鼠抗氧化功能下降,具体机制有待进一步探讨。

关键词: 膳食因素, 高脂膳食, 硒, 抗氧化, 大鼠

Abstract: Objective To explore the effects of selenium on the antioxidant function of rats under high-fat diet and different safe doses of selenium. Methods A total of 60 SPF Wistar rats(6 weeks old, half male and half female)were randomly divided into normal group, normal+0.3 mg/kg Se group, normal+0.9 mg/kg Se group, high-fat group, high-fat+0.3 mg/kg Se group and high-fat+0.9 mg/kg Se group, with 10 rats in each group. The body weight was measured every 2 weeks. The rats were sacrificed after 8 weeks. Serum selenium, glutathione peroxidase(GSH-Px), superoxide dismutase(SOD), and total glutathione(T-GSH)were measured. Statistical analysis was performed using analysis of variance with factorial design. Results In male rats, the activity of GSH-Px was mainly affected by the concentration of selenium(P=0.002). Dietary factors and selenium concentration could affect the activity of SOD(P=0.005, P=0.050). The concentration of T-GSH was mainly affected by dietary factors(P<0.001). In male rats on a high-fat diet, the activity of GSH-PX and serum concentration of T-GSH increased first and then decreased with the elevation of selenium concentration. Conclusion To some extent, low and medium concentrations of selenium promote the antioxidant function of male rats on a high-fat diet, while the antioxidant function decreases with the elevation of selenium concentration.

Key words: Dietary factors, High-fat diet, Selenium, Antioxidation, Rats

中图分类号:

张霁娟,于汉成,王蓝,陈诺,崔书萌,高希宝. 高脂膳食、硒对大鼠抗氧化功能的影响[J]. 山东大学学报 (医学版), 2021, 59(1): 95-101.

ZHANG Jijuan, YU Hancheng, WANG Lan, CHEN Nuo, CUI Shumeng, Gao Xibao. Effects of high-fat diet and selenium concentration on antioxidant function in rats[J]. Journal of Shandong University (Health Sciences), 2021, 59(1): 95-101.

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