山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (3): 25-31.doi: 10.6040/j.issn.1671-7554.0.2016.1372
邓轲,刘毅,盖中涛
DENG Ke, LIU Yi, GAI Zhongtao
摘要： 目的 分析miRNA在肠道病毒71型(EV71)感染间充质干细胞后的表达差异,探讨miRNA在EV71感染中的作用机制。 方法 EV71体外感染脐带间充质干细胞(MSC)建立EV71感染模型,采用基因芯片技术分析EV71感染MSC前后miRNA的差异表达,通过生物信息学分析其相关信号通路。 结果 EV71感染MSC前后有84个miRNA发生了异常变化,主要涉及MAPK、Wnt等信号通路。 结论 MSC细胞对EV71有易感性;EV71通过miRNA调控MAPK、Wnt等信号通路参与EV71感染。
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