间歇性张应力对小鼠破骨细胞TRPV5基因表达的影响

doi:10.6040/j.issn.1671-7554.0.2017.099

山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (11): 22-26.doi: 10.6040/j.issn.1671-7554.0.2017.099

间歇性张应力对小鼠破骨细胞TRPV5基因表达的影响

彭斐¹,宋玲²,戚向敏³,李亚芹²,刁艳菲²,钊守凤⁴,王莉莉⁴,杨芳²,卢恕来²,吕文芳²,郭大伟²

1.潍坊医学院, 山东潍坊 261000;2.青岛市市立医院口腔科, 山东青岛 266071;3.山东大学口腔医学院, 山东济南 250012;4.青岛市市立医院中心实验室, 山东青岛 266071

收稿日期:2017-01-24 出版日期:2017-11-10 发布日期:2017-11-10
通讯作者: 郭大伟. E-mail:dawei_guo@163.com E-mail:dawei_guo@163.com
基金资助:
山东省医药卫生科技发展计划(2013WS0014);青岛市科技计划基础研究项目［(12-1-4-16-(8)-jch)］;青岛市医疗卫生优秀人才培养项目(VDTR2016Y08)

Intermittent tensile stress affects the expression of TRPV5 in murine osteoclasts

PENG Fei¹, SONG Ling², QI Xiangmin³, LI Yaqin², DIAO Yanfei², ZHAO Shoufeng⁴, WANG Lili⁴, YANG Fang², LU Shulai², LÜ Wenfang², GUO Dawei²

1. Weifang Medical University, Weifang 261000, Shandong, China;
2. Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266071, Shandong, China;
3. School of Stomatology, Shandong University, Jinan 250012, Shandong, China;
4. Central Laboratory, Qingdao Municipal Hospital, Qingdao 266071, Shandong, China

Received:2017-01-24 Online:2017-11-10 Published:2017-11-10

摘要/Abstract

摘要： 目的探究在间歇性张应力作用下小鼠破骨细胞内TRPV5的基因表达情况。方法将小鼠破骨细胞分组并施加不同大小的间歇性张应力(A组1 000 με、B组3 000 με、C组5 000 με、D组不做加力处理),通过荧光定量PCR方法检测在应力作用前后TRPV5 的mRNA表达水平。结果对小鼠破骨细胞施加不同程度的间歇性张应力之后,TRPV5基因的表达水平相比对照组(D组)均升高,加力组与对照组之间差异有统计学意义(P<0.001),两两比较结果显示,B组与C组之间差异无统计学意义(P>0.05),其余任意两组之间差异均有统计学意义(P<0.05)。结论间歇性张应力可以促进破骨细胞TRPV5基因的表达,在一定范围内加大力值可提高TRPV5基因的表达。

关键词: 瞬时电位感受器香草精家族受体V, 间歇性张应力, 破骨细胞, 荧光定量PCR

Abstract: Objective To investigate the expression of transient receptor potential vanilloid receptor V(TRPV5)during the differentiation of osteoclasts mediated by intermittent tensile stress. Methods Murine osteoclasts were divided into 4 groups and mediated by different loading stress: group A(1 000 με), group B(3 000 με), group C(5 000 με), and group D(0 με). The mRNA expression of TRPV5 in the 4 groups was detected with fluorescence quantitative PCR before and after the differentiation. Results Compared with group D, the other 3 groups had higher mRNA expression of TRPV5(P<0.001). Pairwise comparison results showed that there was no statistical difference between group B and C(P>0.05),there was statistical difference between any other two groups(P<0.05). Conclusion Intermittent tensile stress promotes the expression of TRPV5 channel gene in osteoclasts within a certain range.

Key words: Intermittent tensile stress, Osteoclast, Transient receptor potential vanilloid receptor 5, Fluorescence quantitative PCR

中图分类号:

R782

彭斐,宋玲,戚向敏,李亚芹,刁艳菲,钊守凤,王莉莉,杨芳,卢恕来,吕文芳,郭大伟. 间歇性张应力对小鼠破骨细胞TRPV5基因表达的影响[J]. 山东大学学报（医学版）, 2017, 55(11): 22-26.

PENG Fei, SONG Ling, QI Xiangmin, LI Yaqin, DIAO Yanfei, ZHAO Shoufeng, WANG Lili, YANG Fang, LU Shulai, LÜ Wenfang, GUO Dawei. Intermittent tensile stress affects the expression of TRPV5 in murine osteoclasts[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 22-26.

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间歇性张应力对小鼠破骨细胞TRPV5基因表达的影响

Intermittent tensile stress affects the expression of TRPV5 in murine osteoclasts

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