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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (9): 41-46.doi: 10.6040/j.issn.1671-7554.0.2014.716

• 基础医学 • 上一篇    下一篇

葡萄籽原花青素对小鼠脂肪肝模型缺血再灌注造成的急性肝损伤的保护作用

李少伟1,2,3, 刘宗正1,2, 刘春霞1,2, 张焱如1,2, 周欢敏1,2   

  1. 1. 内蒙古农业大学生命科学学院, 内蒙古 呼和浩特 010018;
    2. 内蒙古自治区生物制造重点实验室, 内蒙古 呼和浩特 010018;
    3. 内蒙古医科大学基础医学院分子生物学研究中心, 内蒙古 呼和浩特, 010059
  • 收稿日期:2014-10-16 出版日期:2015-09-10 发布日期:2015-09-10
  • 通讯作者: 周欢敏。E-mail:huanminzhou@263.net E-mail:huanminzhou@263.net
  • 基金资助:
    内蒙古自然科学基金重大项目(2010ZD10)

Protective effect of grape seed proanthocyanidins in mouse fatty liver models suffering ischemia-reperfusion injury

LI Shaowei1,2,3, LIU Zongzheng1,2, LIU Chunxia1,2, ZHANG Yanru1,2, ZHOU Huanmin1,2   

  1. 1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia, China;
    2. Inner Mongolia Key Laboratory of Biomanufacture, Hohhot 010018, Inner Mongolia, China;
    3. Center of Molecular Biology, Basic Medical College, Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia, China
  • Received:2014-10-16 Online:2015-09-10 Published:2015-09-10
  • Contact: 周欢敏。E-mail:huanminzhou@263.net E-mail:huanminzhou@263.net

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摘要： 目的 探讨葡萄籽原花青素对脂肪肝缺血再灌注(IR)损伤小鼠模型的保护作用。方法 将30只C57BL/6小鼠随机分为3组。喂食蛋氨酸胆碱缺乏高脂饲料3周后进行非酒精性脂肪肝造模,实验分3组:假手术组、对照组(肝脏缺血再灌注前灌服蒸馏水)、花青素缺血再灌注组(肝脏缺血再灌注前灌服原花青素)。再灌注6 h后处死小鼠,检测血清中丙氨酸转氨酶(AST)和天冬氨酸转氨酶(ALT)含量,观察肝脏组织形态学变化,分析肝脏组织损伤程度,巨噬细胞浸润程度,炎性因子TNF-α、IL-6、iNOS、INFγmRNA表达水平和肝脏组织内p38蛋白表达水平。结果 与对照组相比较,花青素组血清ALT和AST水平较低(P<0.05),巨噬细胞浸润和凋亡细胞明显减少,炎性因子mRNA的表达明显降低。蛋白水平p38基本无变化,磷酸化的p38表达显著升高。结论 花青素能够减轻脂肪肝小鼠肝脏IR损伤,其机制可能与抑制肝脏再灌注后氧化应激反应和炎症反应,促进p-p38的表达升高有关。

关键词: 脂肪肝, 氧化应激, 炎症反应, 缺血再灌注损伤, 葡萄籽原花青素

Abstract: Objective To explore the protective effects of grape seed proanthocyanidins (GSP) on liver ischemia-reperfusion (IR) in mouse fatty liver models and the possible mechanism. Methods Thirty C57BL/6 mice were randomly divided into 3 groups: sham-operated group (SO group), IR group (mice with steatotic liver treated with distilled water by gavage just before ischemia reperfusion) and GSP group (mice with steatotic liver gavaged with 500 mg/kg GSP). The mice were then sacrificed 6h after reperfusion, and serum and liver samples were collected. The ALT and AST levels in serum were determined and histiological damage on the liver was evaluated. The infiltration of F4/80 positive macrophage cells was detected using immunohistochemistry. The mRNA expressions of TNF-α, IL-6, iNOS, and INF-γ were assayed using real-time reverse transcription PCR. The expression of p38 protein was detected using Western blotting. Results Compared with the IR group, mice in the GSP group exhibited lower levels of serum ALT and AST (P<0.05), and milder histological damage. Compared with the IR group, mice in the GSP group had reduced infiltration of macrophages and lower mRNA expressions of TNF-α, IL-6, iNOS and INF-γ in the liver tissues (P<0.05). The p38 expressions in the three groups had no difference, but the phosphorylated p38 (p-p38) expression in the GSP group was statistically higher than in the other two groups. Conclusion GSP can alleviate steatotic liver IR injury by inhibiting oxidant stress and inflammatory response induced by reperfusion.

Key words: Inflammatory, Grape seed proanthocyanidins, Oxidant stress, Reperfusion injury, Fatty liver

中图分类号: 

  • R574
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