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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (3): 17-21.doi: 10.6040/j.issn.1671-7554.0.2014.779

• 基础医学 • 上一篇    下一篇

CaSki细胞内HPVL1蛋白的表达

杜义江, 肖长义, 叶红, 汤桂成, 黎家华   

  1. 三峡大学医学院组织学与胚胎学教研室, 湖北 宜昌 443002
  • 收稿日期:2014-11-03 修回日期:2015-01-23 出版日期:2015-03-10 发布日期:2015-03-10
  • 通讯作者: 肖长义, E-mail:xiaochy@ctgu.edu.cn E-mail:xiaochy@ctgu.edu.cn
  • 基金资助:
    湖北省自然科学基金(2011CDC002)

Expression of human papillomavirus late capsid protein 1 in CaSki cells

DU Yijiang, XIAO Changyi, YE Hong, TANG Guicheng, LI Jiahua   

  1. Department of Histology and Embryology, Medicine College of China Three Gorges University, Yichang 443002, Hubei, China
  • Received:2014-11-03 Revised:2015-01-23 Online:2015-03-10 Published:2015-03-10

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摘要： 目的 了解含人乳头瘤病毒(HPV)基因组的宫颈癌CaSki细胞中HPV晚期衣壳蛋白1(L1)的表达。方法 以多价HPV L1小鼠单克隆抗体做探针, 采用ELISA、免疫组化、Western blotting等方法, 检测CaSki细胞内L1蛋白的表达;采用RT-PCR法检测细胞内L1 mRNA的表达。结果 CaSki细胞呈HPV L1阳性反应, 显示细胞内有HPV L1蛋白存在;CaSki细胞内有L1 mRNA存在。结论 包含HPV16混合基因组的CaSki细胞可以表达L1蛋白。

关键词: CaSki细胞, 人乳头瘤病毒, 晚期衣壳蛋白1, 宫颈癌

Abstract: Objective To investigate the expression of human papil1omavirus (HPV) late capsid protein 1 (L1) in CaSki cells. Methods Using polyvalent HPV L1 mice monoclonal antibody as the probe, the L1 protein expression in CaSki cells was investigated by ELISA, immunohistochemistry and Western blotting. L1 mRNA expression was detected by RT-PCR. Results HPV L1 was strong positive in CaSki cells, indicating that L1 protein was expressed in CaSki cells. L1 mRNA was expressed in CaSki cells. Conclusion CaSki cells integrated HPV 16 DNA genome can express L1 protein.

Key words: Caski cells, Human papillomavirus, Uterine cervix cancer, Late capsid protein 1

中图分类号: 

  • R737.33
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