机械应力作用下促进骨不连愈合的疗效

doi:10.6040/j.issn.1671-7554.0.2014.732

摘要/Abstract

摘要： 目的观察机械应力作用下促进大鼠骨不连愈合的效果,为机械应力促进骨不连断端骨折愈合提供分子水平的理论依据.方法选取6月龄雌性SD大鼠,采用自制的可调式外固定架建造大鼠骨不连模型,并通过骨不连断端影像学评分,确定骨不连模型的建立.待模型建成后,随机分为实验组和对照组,每组30只,其中实验组骨折断端加压,对照组骨折断端不作处理,进一步对断端影像学和组织学钙钴法染色、成骨因子(BMP、VEGF)扩增检测.结果实验组加压12周后,影像学评分较同组4、8周及对照组差异有统计学意义(P<0.05).钙钴法显示,在不同时间点实验组骨不连断端成骨细胞数量明显增多,与对照组相比差异有统计学意义(P<0.05).实验组BMP、VEGF在不同时期的基因扩增明显高于对照组(P<0.05).结论机械应力能有效促进骨不连断端成骨细胞数量的增多及成骨因子的扩增,并有效促进骨不连断端的愈合.

关键词: 骨折, 大鼠模型, 骨不连, 机械应力, 成骨因子

Abstract: Objective To observe the therapeutic effects of mechanical stress in treating nonunion in animal models. Methods Animal models of nonunion were built using self-adjustable external fixation. The models were then randomly divided into the experimental group and control group, with 30 animals in either group. The experimental group received axial pressure on the fracture end, while the control group received no pressure. Then nonunion was imaged and stained, and osteogenetic factors such as bone morphogenetic protein (BMP) and vascular endothelial growth factor (VEGF) were detected. Results There were statistical differences between the experimental group and control group (P<0.05). Calcium cobalt staining revealed that more osteoblasts were generated, and more amplification of BMP and VEGF were observed in the experimental group at different time points (both P<0.05). Conclusion Mechanical stress can promote the growth of osteoblasts and amplification of osteogenetic factors in the nonunion, and effectively accelerate its healing.

Key words: Mechanical stress, Rat model, Fracture, Nonunion, Osteogenetic factors

中图分类号:

R687.1

李尚志, 刘海春, 武文亮, 曹聪, 陈允震. 机械应力作用下促进骨不连愈合的疗效[J]. 山东大学学报（医学版）, 2015, 53(2): 22-26.

LI Shangzhi, LIU Haichun, WU Wenliang, CAO Cong, CHEN Yunzhen. Curative effect of mechanical stress in the treatment of nonunion[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 22-26.

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