罗勒多糖对乳腺癌细胞系MDA-MB-231侵袭的影响

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (9): 50-54.

罗勒多糖对乳腺癌细胞系MDA-MB-231侵袭的影响

石焕1,2，邵倩倩1，孙锦堂1，宋丙凤1，吕静1,3，何影1，张韵1，曲迅1

1. 山东大学齐鲁医院临床基础研究所，济南 250012；
2. 山东省肿瘤医院内科三病区，济南 250117；
3. 青岛大学医学院附属医院肿瘤科，山东青岛 266071

收稿日期:2013-06-24 出版日期:2013-09-10 发布日期:2013-09-10
通讯作者: 曲迅，E-mail：quxun@sdu.edu.cn

Effect of basil polysaccharide on the invasiveness of MDA-MB-231 cells

SHI Huan1,2, SHAO Qian-qian1, SUN Jin-tang1, SONG Bing-feng1, Lv Jing1,3, HE Ying1, ZHANG Yun1, QU Xun1

1. Institute of Clinical and Basic Medicine Research, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Third Ward of Internal Medicine, Shandong Tumor Hospital, Jinan 250117, China;
3. Oncology Ward, the Affiliated Hospital of Medical College of Qingdao University, Qingdao 266071, China

Received:2013-06-24 Online:2013-09-10 Published:2013-09-10

摘要/Abstract

摘要：

目的观察罗勒多糖对乳腺癌细胞系MDA-MB-231侵袭性的影响。方法将体外培养的MDA-MB-231细胞分为实验组和对照组。实验组细胞用不同浓度罗勒多糖（100、300μg/mL）处理，对照组不加任何刺激因子。Transwell小室检测细胞侵袭能力，RT-PCR、Western blotting方法检测与侵袭相关的基质金属蛋白酶2（MMP-2）、基质金属蛋白酶9（MMP-9）和膜型基质金属蛋白酶1（MT1-MMP）的表达。结果 ①罗勒多糖可抑制MDA-MB-231细胞的侵袭力（P<0.01）；②罗勒多糖抑制MT1-MMP分子的mRNA表达（P<0.05）及下调其蛋白表达水平。③罗勒多糖对MMP-2及MMP-9分子的mRNA表达无影响。结论罗勒多糖可通过下调MT1-MMP的表达抑制MDA-MB-231细胞的侵袭。

关键词: 罗勒多糖；肿瘤侵袭；膜型基质金属蛋白酶-1；乳腺肿瘤

Abstract:

Objective To investigate the effect of basil polysaccharide on the invasiveness of MDA-MB-231 cells. Methods MDA-MB-231 cells cultured in vitro were divided into the experimental group and control group. The control group was not treated, while the experimental group was treated with 100μg/mL or 300μg/mL basil polysaccharide. The invasiveness of the cells was detected with transwell assay. The expressions of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and membrane-type 1 matrix metalloproteinase (MT1-MMP) were tested with RT-PCR and Western blotting. Results Basil polysaccharide suppressed the invasion of MDA-MB-231 cells (P<0.01), decreased the mRNA expression of MT1-MMP (P<0.05), but had no effect on the mRNA expression of MMP-2 and MMP-9. Conclusion Basil polysaccharide inhibits the invasiveness of MDA-MB-231 cells by reducing the expression of MT1-MMP.

Key words: Basil polysaccharide; Tumor invasion; Membrane-type 1 matrix metalloproteinase; Breast neoplasm

中图分类号:

R73-37

石焕1,2，邵倩倩1，孙锦堂1，宋丙凤1，吕静1,3，何影1，张韵1，曲迅1. 罗勒多糖对乳腺癌细胞系MDA-MB-231侵袭的影响[J]. 山东大学学报(医学版), 2013, 51(9): 50-54.

SHI Huan1,2, SHAO Qian-qian1, SUN Jin-tang1, SONG Bing-feng1, Lv Jing1,3, HE Ying1, ZHANG Yun1, QU Xun1. Effect of basil polysaccharide on the invasiveness of MDA-MB-231 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(9): 50-54.

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