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山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (8): 17-21.

• 基础医学 • 上一篇    下一篇

白藜芦醇对高脂饮食去卵巢肥胖大鼠脑组织炎症反应及海马Aβ1-42水平的影响

苗玉连,武传龙,刘金波,张晓黎   

  1. 山东大学齐鲁医院内分泌科, 济南 250012
  • 收稿日期:2013-01-14 出版日期:2013-08-10 发布日期:2013-08-10
  • 通讯作者: 张晓黎, E-mail:sallyzh66@sina.com
  • 基金资助:

    山东省优秀中青年科学家科研奖励基金(BS2009YY038);山东大学自主创新基金(2012TS162)

Effects of resveratrol on brain inflammation and the level of Aβ1-42 in hippocampus from ovariectomized obese rats fed with a high-fat diet

MIAO Yu-lian, WU Chuan-long, LIU Jin-bo, ZHANG Xiao-li   

  1. Department of Endocrinology, Qilu Hosipital of Shandong University, Jinan 250012, China
  • Received:2013-01-14 Online:2013-08-10 Published:2013-08-10

摘要:

目的   探讨白藜芦醇对高脂饮食去卵巢肥胖大鼠海马组织炎症反应及β淀粉样蛋白1-42(Aβ1-42)水平的影响。方法   健康3月龄Wistar雌性大鼠30只随机分为假手术普通饮食组(SHAM组)、去卵巢高脂饮食组(OH组)、白藜芦醇[40mg/(kg·d)-1]干预组(OHR组)。相应干预3个月后检测大鼠血清雌二醇(E2)、胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平,RT-PCR法检测海马组织中白细胞介素6(IL6)及肿瘤坏死因子α(TNF-α) mRNA 的表达,ELISA法检测海马匀浆IL-6、TNF-α与β淀粉样蛋白1-42(Aβ1-42)表达。结果   OH组大鼠血清E2水平较SHAM组明显降低(P<0.01),OHR组大鼠血清中E2水平较OH组显著升高(P<0.05);OH组大鼠血清TC、LDL-C含量较SHAM组升高(P<0.01),OHR组大鼠血清TC含量较OH组降低(P<0.01);OH组大鼠海马组织IL-6、TNF-α mRNA及IL-6、TNF-α、Aβ1-42蛋白含量较SHAM组明显升高(P<0.01),OHR组IL-6、TNF-α mRNA及Aβ1-42蛋白含量较OH组明显降低(P<0.05)。结论   白藜芦醇能减轻高脂饮食去卵巢肥胖大鼠海马组织中Aβ1-42的沉积,其机制可能与白藜芦醇升高大鼠血清E2水平、降低血清TC含量、改善肥胖相关大脑炎症反应有关。

关键词: 白藜芦醇;肥胖;海马;炎症反应;Aβ1-42

Abstract:

Objective   To explore the effects of resveratrol on the neuroinflammation and the level of Aβ1-42 in hippocampus from ovariectomized obese rats fed with a highfat diet. MethodsThirty female Wistar rats aged 3 months were randomly divided into three groups: the sham operation fed with low-fat diet (SHAM) group; the ovariectomy fed with high-fat diet(OH) group; the ovariectomy plus highfat diet treated with resveratrol [40mg/(kg·d)-1] (OHR) group. After three months of administration, the rats blood was collected from arteria cruralis to detect the serum concentration of total TC, TG, LDLC, HDLC and E2; the expression of IL6 and TNFα mRNA in hippocampus was determined by realtime PCR; the levels of IL6, TNFα and Aβ142 protein in hippocampal tissues were observed by ELISA. ResultsThe level of serum E2 was significantly lower in the OH group than that in the SHAM group(P<0.01). However, rats in the OHR group had a higher level of serum E2 than rats in the OH group (P<0.05); The levels of serum TC and LDLC in the OH group were higher than those in the SHAM group (P<0.01), and rats in the OHR group had a lower level of serum TC(P<0.01); Expression of IL6, TNFα mRNA and IL6, TNFα, Aβ142 proteins in the OH group significantly increased compared with that in the SHAM group (P<0.01). Nevertheless, rats in the OHR group showed significantly lower expression of IL6, TNFα mRNA and IL6, TNFα, Aβ142 proteins rats in the OH group (P<0.05). ConclusionResveratrol can attenuate the accumulation of Aβ142 in hippocampus from ovariectomized obese rats fed with a highfat diet. The mechanism may be associated with its increasing the level of E2, decreasing the content of TC and ameliorating the obesityrelated brain inflammatory responses.

Key words: Resveratrol; Obesity; Hippocampus; Inflammatory responses; Aβ1-42

中图分类号: 

  • R589
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