GLP-1对3T3-L1前脂肪细胞分化过程中FABP-4、CPT-1A水平的影响

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (8): 22-27.

GLP-1对3T3-L1前脂肪细胞分化过程中FABP-4、CPT-1A水平的影响

杨俊朋1,2，侯新国1,2，宋君1,2，武传龙1,2，李文娟1,2，龚蕾1,2，闫飞1,2，王令舒1,2，陈丽1,2

山东大学 1.内分泌代谢病研究所； 2.齐鲁医院内分泌科，济南 250012

收稿日期:2013-01-08 出版日期:2013-08-10 发布日期:2013-08-10
通讯作者: 陈丽, E-mail: chenli3@medmail.com.cn
基金资助:
2011年山东省医药卫生科技发展计划重点项目(2011HD005)；国家科技支撑计划(2009BAI80B04)；山东省自然科学基金面上项目(ZR2012HM014)；中华医学会糖尿病分会重点项目(07020470055)；山东省科技攻关国际科技合作项目(2010GHZ20201)；济南市科技局科技明星项目(20100318)；山东大学自主创新基金(2009TS054)；国家自然科学基金(81100617)；济南留学人员创业计划(20110407)

Effect of glucagon-like peptide-1 on the levels of FABP-4 and CPT-1A during the process of 3T3-L1 preadipocyte differentiation

YANG Jun-peng1,2, HOU Xin-guo1,2, SONG Jun1,2, WU Chuan-long1,2, LI Wen-juan1,2, GONG Lei1,2, YAN Fei1,2, WANG Ling-shu1,2, CHEN Li1,2

1. Institute of Endocrinology and Metabolism; 2. Department of Endocrinology,
Qilu Hospital of Shandong University, Jinan 250012, China

Received:2013-01-08 Online:2013-08-10 Published:2013-08-10

摘要/Abstract

摘要：

目的探讨胰高糖素肽-1(GLP-1)对3T3-L1前脂肪细胞分化过程中游离脂肪酸结合蛋白4(FABP-4)、肉碱脂酰转移酶1A(CPT-1A)表达水平的影响。方法采用传统“鸡尾酒”法诱导3T3-L1前脂肪细胞分化，并在分化过程中加入1×10-8mol/L GLP-1进行干预。在诱导分化的第6、8天，用油红O染色检测细胞内脂质聚集，RT-PCR及Western blotting法检测FABP-4、CPT-1A的mRNA及蛋白水平。结果 ①在诱导分化第6天，大约40%的细胞出现脂滴聚集，第8天时大约80%的细胞已分化成熟；②油红O染色结果显示，与对照组相比，GLP-1组脂质总量在分化第6天略有增加，在分化第8天轻度下降(P>0.05)；③RT-PCR结果显示, GLP-1组FABP-4的mRNA水平在分化第6、8天都明显增加；CPT1-A的mRNA水平在分化第8天增加明显(P<0.05)；④Western blotting结果显示,GLP-1显著增加了FABP-4的蛋白表达(P<0.05)。对照组CPT-1A的蛋白水平在分化第6、8天无显著变化；而GLP-1组CPT-1A蛋白水平显著增加，尤其在分化第8天，与对照组相比增加了3.57倍(P<0.01)。结论在3T3L1前脂肪细胞分化过程中，GLP-1显著促进了FABP-4和CPT-1A的mRNA及蛋白水平，提示GLP-1同时促进脂肪细胞对游离脂肪酸的吸收及分解，能有效改善游离脂肪酸介导的胰岛素抵抗。

关键词: GLP-1；前脂肪细胞分化；FABP-4；CPT-1A

Abstract:

Objective To examine the effect of glucagon-like peptide-1 (GLP-1) on the levels of fatty acid binding protein 4 (FABP-4) and carnitine palmitoyltransferase 1A (CPT-1A) during 3T3-L1 preadipocyte differentiation process. Methods In this paper, the traditional “Cocktail method” was adopted to induce the 3T3-L1 preadipocyte differentiation and 1×10-8 mol/L GLP-1 was added to the medium. At the 6th day and the 8th day of differentiation, Oil Red O staining was used to examine the lipid accumulation; RT-PCR and Western blotting were employed to test the levels of FABP-4 and CPT-1A. Results ①It is found that about forty percent of the cells appeared lipid droplets at the 6th day; about eighty percent of the cells had differentiated to mature adipocytes at the 8th day. ②The results of Oil Red O staining showed that the lipid accumulations in GLP-1 group were slightly increased at the 6th day while modestly decreased at the 8th day (both P>0.05), as compared to the control group. ③RTPCR results indicated that the mRNA level of FABP-4 in GLP-1 group was significantly increased at the 6th day and the 8th day. And the mRNA level of CPT-1A was also enhanced at the 8th day (P<0.05). ④ Western blotting results showed that the protein expression of FABP-4 in GLP-1 group was improved markedly at the 6th day and the 8th day (P<0.05). The protein level of CPT-1A in control group was not changed significantly. While it was greatly increased in the cells treated with GLP-1, which was increased 3.57 times as compared to control group at the 8th day (P<0.01). Conclusion GLP-1 can significantly promote the expressions of FABP-4 and CPT-1A during the process of 3T3-L1 preadipocyte differentiation, which indicates that GLP-1 can simultaneously promote the fatty acid absorption and decomposition and it has played an important role in improving the free fatty aicd induced insulin resistance.

Key words: Glucagon-like peptide-1 (GLP-1); Preadipocyte differentiation; FABP-4; CTP-1A

中图分类号:

R589.2

杨俊朋1,2，侯新国1,2，宋君1,2，武传龙1,2，李文娟1,2，龚蕾1,2，闫飞1,2，王令舒1,2，陈丽1,2. GLP-1对3T3-L1前脂肪细胞分化过程中FABP-4、CPT-1A水平的影响[J]. 山东大学学报(医学版), 2013, 51(8): 22-27.

YANG Jun-peng1,2, HOU Xin-guo1,2, SONG Jun1,2, WU Chuan-long1,2, LI Wen-juan1,2, GONG Lei1,2, YAN Fei1,2, WANG Ling-shu1,2, CHEN Li1,2. Effect of glucagon-like peptide-1 on the levels of FABP-4 and CPT-1A during the process of 3T3-L1 preadipocyte differentiation[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(8): 22-27.

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